Basic Vector Information
- Vector Name:
- pTX-NLS-Cre
- Antibiotic Resistance:
- Ampicillin
- Length:
- 11491 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Linkner J, Nordholz B, Junemann A, Winterhoff M, Faix J.
pTX-NLS-Cre vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTX-NLS-Cre vector Sequence
LOCUS 40924_44544 11491 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pTX-NLS-Cre, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11491)
AUTHORS Linkner J, Nordholz B, Junemann A, Winterhoff M, Faix J.
TITLE Highly effective removal of floxed Blasticidin S resistance
cassettes from Dictyostelium discoideum mutants by extrachromosomal
expression of Cre
JOURNAL Eur. J. Cell Biol. (2011) In press
PUBMED 22154549
REFERENCE 2 (bases 1 to 11491)
AUTHORS Linkner J, Faix J.
TITLE Direct Submission
JOURNAL Submitted (16-NOV-2011) Institute for Biophysical Chemistry,
Hannover Medical School, Carl-Neuberg-Str. 1, Hannover 30625,
Germany
REFERENCE 3 (bases 1 to 11491)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 11491)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Eur. J.
Cell Biol. (2011) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(16-NOV-2011) Institute for Biophysical Chemistry, Hannover Medical
School, Carl-Neuberg-Str. 1, Hannover 30625, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..11491
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter complement(12..30)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(51..67)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(75..91)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(99..129)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(144..165)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(453..1041)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1215..2072)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2073..2177)
/label=AmpR promoter
rep_origin complement(2203..2658)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 2800..2816
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 2826..2844
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 3194..3217
/codon_start=1
/label=8xHis
/note="8xHis affinity tag"
/translation="HHHHHHHH"
CDS 3233..3253
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 3251..4279
/codon_start=1
/label=Cre
/note="site-specific recombinase"
/translation="VSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLQ
DGD"
CDS 5114..5905
/codon_start=1
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
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