LOCUS       40924_44544       11491 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTX-NLS-Cre, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Linkner J, Nordholz B, Junemann A, Winterhoff M, Faix J.
  TITLE     Highly effective removal of floxed Blasticidin S resistance 
            cassettes from Dictyostelium discoideum mutants by extrachromosomal 
            expression of Cre
  JOURNAL   Eur. J. Cell Biol. (2011) In press
  PUBMED    22154549
REFERENCE   2  (bases 1 to 11491)
  AUTHORS   Linkner J, Faix J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2011) Institute for Biophysical Chemistry, 
            Hannover Medical School, Carl-Neuberg-Str. 1, Hannover 30625, 
            Germany
REFERENCE   3  (bases 1 to 11491)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11491)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur. J. 
            Cell Biol. (2011) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-NOV-2011) Institute for Biophysical Chemistry, Hannover Medical 
            School, Carl-Neuberg-Str. 1, Hannover 30625, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(12..30)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(51..67)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(75..91)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(99..129)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(144..165)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(453..1041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1215..2072)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2073..2177)
                     /label=AmpR promoter
     rep_origin      complement(2203..2658)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2800..2816
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2826..2844
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3194..3217
                     /codon_start=1
                     /label=8xHis
                     /note="8xHis affinity tag"
                     /translation="HHHHHHHH"
     CDS             3233..3253
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3251..4279
                     /codon_start=1
                     /label=Cre
                     /note="site-specific recombinase"
                     /translation="VSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLQ
                     DGD"
     CDS             5114..5905
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
       61 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      121 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      181 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      241 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      301 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      361 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      421 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      481 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      541 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      601 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      661 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
      721 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      781 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      841 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
      901 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      961 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1021 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1081 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1141 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1201 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1261 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1321 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     1381 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1441 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1501 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     1561 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     1621 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1681 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1741 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1801 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     1861 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1921 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1981 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2041 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2101 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2161 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     2221 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     2281 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     2341 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     2401 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     2461 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     2521 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     2581 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     2641 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     2701 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     2761 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
     2821 gcgcgtaata cgactcacta tagggcgaat tggagctggt cgactagata aaaaaaattt
     2881 ttatttattt ttattatttt gaattaaata gattacaaat gattacaaat taattaatcc
     2941 catcaaatct ttaaaaaaaa aaatggttta aaaaaacttg ggttggttaa ttattatttg
     3001 aaaattttaa aacccaaatt aaaaaaaaaa aatgggattc aaaaattttt tttttttttt
     3061 tttttttttt tttttttttt tttttttttt tcagattgca taaaaagatt tttttttttt
     3121 ttttttctta tttcttaaaa caaataaatt aaattaaata aaaaataaaa atcagatcca
     3181 agcttaaaaa atgcatcatc atcatcatca tcatcatgat atcggtacca tgccaaaaaa
     3241 aaagaggaag gtgtccaatt tactgaccgt acaccaaaat ttgcctgcat taccggtcga
     3301 tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc gccaggcgtt
     3361 ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa
     3421 gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt atcttctata
     3481 tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc agctaaacat
     3541 gcttcatcgt cggtccgggc tgccacgacc aagtgacagc aatgctgttt cactggttat
     3601 gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt
     3661 cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgatc gctgccagga
     3721 tatacgtaat ctggcatttc tggggattgc ttataacacc ctgttacgta tagccgaaat
     3781 tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt taatccatat
     3841 tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta gcctgggggt
     3901 aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc cgaataacta
     3961 cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca gccagctatc
     4021 aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg gcgctaagga
     4081 tgactctggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg
     4141 agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg gctggaccaa
     4201 tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg caatggtgcg
     4261 cctgctgcaa gatggagatt agaataactc gagatgcatt agaacaaaaa ttattatcag
     4321 aagaagattt aaattaatct agacgatccc ccgggctgca ggaattcaga ggtttatttt
     4381 taaaaattac aaaaactaaa aagaaaaata aaaaggaaaa atcttattaa tctgaaaatt
     4441 acagatttgc cccaatttaa agaaaaatta tccaaaataa tcaatcaaca acgatatctt
     4501 tttgatagat atttataaaa actttatctt ttttattttt tcaaagttgc gcaaaataat
     4561 aaaattaaaa taaatataaa aactgtaaaa agaaaaaaaa aagtgtaaag ggttattaat
     4621 tatttaatta ttattcactt tttgtaatta tttttttatt ttgaagaata atgatggata
     4681 ttttatataa aaaaaaaaga gatatgaaaa aataataatt ataaaaaaaa aaaaaaaaat
     4741 agaattttaa agtttttgta caaatggatg attttttttt tttttttttt cctaaataat
     4801 tcaagtaata aacaaagaat tgatattctg atgcctaatt aaaaaagaaa tttttaaata
     4861 aaaaatgggt tttttttaag taaagttatt tgaaattgat tgaaattttc aaacctaggg
     4921 tggtttttcg ctttaaaatt gggattttat ttttattttt ttatattttt tattttttat
     4981 tttttttttt tttgaggttt ctgagattat aaaatgaaat tttttttttt tttttaatta
     5041 attcaaaaaa taatcaaata aataaatata atataaaatg gatggtgaag atgttcaagc
     5101 tggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     5161 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     5221 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     5281 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     5341 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     5401 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     5461 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     5521 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     5581 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     5641 gcgcatgccc gacggcgagg atctcgtcgt gactcatggc gatgcctgct tgccgaatat
     5701 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     5761 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     5821 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     5881 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     5941 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6001 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6061 ctggagttct tcgcccaccc cgggctcgat cccctcgcga gttggttcag ctgctgcctg
     6121 aggctggacg acctcgcgga gttctaccgg cagtgcaaat ccgtcggcat ccaggaaacc
     6181 agcagcggct atccgcgcat ccatgccccc gaactgcagg agtggggagg cacgatggcc
     6241 gctttggtcc cggatcagct ttatcttttt gattttcata ctctttggct gttttacatc
     6301 taatgcatat atttatcttc accattcaaa atgtatctgt aaataataaa ttttttaaat
     6361 atatttgatt tttttgatag aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaatta
     6421 aaatacctta ataattatta agatttaaaa aaaaaaaatt aaaaaaaaaa gaaaatatta
     6481 gattattaca acaatttgtt tttttattat ttattaaata atttaaaaac attttctgtg
     6541 aacaattgat ggaccctcag gaagatcgca ctccagccag ctttccggca ccgcttctgg
     6601 tgccggaaac aggcaaagcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     6661 tcggtgcggg gatctcgaag aaaaatcgag cataatatcg tcaagcttga tgtcagaatt
     6721 aatataacca aaaacatcac caacaaacga agtttggtga gaaatttgaa taaagatacc
     6781 ttttaaaaaa ttattatcaa tgagatatga gtaattatca ataaaccgtt ggagtgaaat
     6841 tttaccagct gatagtgaac aaaaatatgg aaactcttta agtgaatcaa tatttggaaa
     6901 tggtggatca gaagagtcga tatagatttg ggttatggta ttggaaatgt attgatttaa
     6961 agatttcaaa aggttggctt cagaaatttc atttgaggat gtcctttctg ttacagcttc
     7021 agtataaact ttaacataaa ttgattttaa tagattggga tactgggatg attctttttt
     7081 ttttttatct gcatttgaaa tcatcatggt gaggtttgaa aggacacctt caagttgatc
     7141 gcttacctgt tgaacttgat ttgttttaaa tagttcacca tcaacaggga caggcttttt
     7201 aacaaatttg agagcattaa caacgtgata ttgaacttgt gaaacaaggt tataagtaga
     7261 gctgttagct aaatcaagac gaaaaatttt ccccaatttg tttttaaatt cttcgcttgc
     7321 gccaattaaa actccagcta atgaatatga attgtgaatg ttttgaactc ctaaaatttc
     7381 aacattttgt ttttttggtg tgttgttcga tggaggagca attttccttt tgtttgttgt
     7441 tgttgttgtt gttgacattt gaaattacaa tgaatgtttt tcggttttta ttttttactt
     7501 tttatttgat tttttttttt tttttttttt tttttttttt tttaaaattt tttttttttt
     7561 tttttcaaat tttttttttt ttaaaaaaaa aaaaattcat ttttgtattt aaattatttt
     7621 ataaataaaa aatgatagta gtagaaattg ttttttggac aagaatcgta aagtcaaaat
     7681 attcagttga tgatgaaatc tttaaatatt tggttaatag agcaaaagtt gtgtataaca
     7741 attcagatta taaaacacta tctcaaactg atttagagaa atgggcgatt caaaacactc
     7801 acagaacaat aacttcaatt aaaaatcgtt tttcagtcaa aaagattgga gatgaagaaa
     7861 agttatttag aatttcaaaa aatggtaaaa tccatcttta tacatgttca ttttaattta
     7921 ttttaactaa ttttttaaat taggggagct tattgttctt aatgaattag aattcgataa
     7981 ttttcatatt aaagaaggaa agcatttaag aaaatcaaaa atgtttaatc atatcaaaga
     8041 cagtggttat tatgcaacaa acgaagaaat tgaaattttt cttgaaagct gcactctatg
     8101 taaggaaatt actgcacaaa ccaaacgtaa ttcatataaa aaaagaaata taattaataa
     8161 attaccggaa gaagaagaag aagaagaaga agaagaagaa gaagaagaag aagaagaaga
     8221 acaagaagaa gaagtagaaa aaccgacaat atcggaagaa gaagaagaag aaacacctgc
     8281 agtatcagaa gaagaaaaag aagaggaaga acaagaagaa gataaagaaa aagataaaga
     8341 aaaaaaaata gaagaagata cagaaacagg aaaaaaaaaa gatgaagtta tgaatgaacg
     8401 tcaagatggt attgaaatta gtgaaaaaac aaatgataaa ccaacagaaa aaacaaaagt
     8461 taaaaagaat gtttcacgag tcaaagatat taatcttcat aaaaaaggaa atcctattac
     8521 cccaaaaaaa ataaaaaatt tcagtcaaca acaattacgt ttagcttctg gtgtttcaaa
     8581 tgaaaaaaga gcaataatta ctcttaaaaa ataataaaac tttatttaat taaaaaaata
     8641 attaaaaaga aaattaaata tatattaatt tttaaattat ttgaaatgtt tttttcttga
     8701 gttactgact tttccatacc gttatttgag ccatcaagct ttggattgta aattgttgat
     8761 aaaaattcag aatcagatgc accatacttt ggagtacact ttgttgtttt aaaatcaaga
     8821 ttgttttgaa tgttttcatt cttacaatag tggcacaatg gatctgtaca tgtaaatcta
     8881 ttggatttct cttgtgtaat gtttgtaata tgatatacat tttcaaattt tgaacaaatg
     8941 ttgaattttt caataattgt tttactattt tttgtacaat ccattgaaag agaagtaatt
     9001 tcaacaaagc tgtcttccac tctaaacaaa taaccatcat cagtttcatt atattcttta
     9061 taaagaatag actctccaaa ctcgtcatta atacattcat taagcttaat taagtttgtt
     9121 aaattacgaa aattgttaca tcttgaatta tttgtttcaa tgtcatcata aaaaagcgca
     9181 tgagtactat ttgtttcaac tcttccatga atattacatt catcataaat tacggcgcct
     9241 gtcattgact tttcaacaca gttttctttt tgatacataa caataagaag ttgaaaatat
     9301 tttacttcaa tctgatcatt ttgaagaaca gaagattttg atacaataaa aaaaaaaaga
     9361 aaaaataata acgttgttga gtacattata tacaatgaaa ataataaatg aacaaaaaat
     9421 aattttttat tataatatat taaaccccac cccacaacca cacctaaaaa aaagttaata
     9481 cttttttaaa tataattttt ttaatagtta ttttaatacg tttttaacat aaaatatatt
     9541 ttaatttttt ttttaatttt ttagaaaatt tttttttcat aattatttta atactttttt
     9601 acaaaaaata tttttaaaat tttaaaaatt ttttttttat aattatttta atagcttaat
     9661 tacaaaaaca atttaataat ttttttaatt tttttttcat aatattttta atagcttttt
     9721 tacaaattat ttttacgaaa atttttgtta tattttttta taattatttt aatacatttt
     9781 taacattaaa taattatata attttttaat acaataatta cagttataaa ttcatattat
     9841 ttaaatattt ttaaatgagt ttatttagaa attaagttta tattttattt aattagtttt
     9901 gactctcaga atgtatagac actttttaga acactgtgca caaagatgaa gtttacattc
     9961 ttggttcaaa ttacatttag ttccgttctt actacacatg gcacacttct tgttgattac
    10021 cgaatcattg aaacatggag ggcaaagttt agaattaata acaacattat atatgtttat
    10081 gtttattcct aaacaattat agcaaatatt gttttgacat ccatctcttg tacatgaaat
    10141 atataaattt tttccttgac acaaagaatg acaattaata cactttactt ctaagagaga
    10201 ataattttct ggatttgaaa gggcatctgt tgaagaaact aaaaagaaat caattttgga
    10261 agaagtttta ttgacttctt ctttaataaa cttttttaaa tcggcaattt cattggaatt
    10321 tatttgggta gattcggatt gaattttgta ataattgcta tttacttttt cgaagttatt
    10381 gaaaaaaggt tttaaattaa atatatcttg tgttgaaaat tcttttaatg aaagtatttg
    10441 ttttaaagca gtactttgac tttgaatatg aattaaaaga tttttggttt cttcataatt
    10501 aattagcgat ggaaaatatc taaatacaag aatgcttgtt atatgaacaa tttttccttg
    10561 cgaattaatt ctaattgagt aatgaataaa ggaatcgcta aaaggtgttg agtctacaaa
    10621 aacagaagga ggtttaatta acaacgccat attattctga tcgttttcat taaaaaaaca
    10681 atatttatct actaatgaaa atcgattaat aatataatta tctaaattat taaaagtctc
    10741 tttatctttt aaaagaacaa gtgtatgacg agaaacttga tcaaggcagt aatgtgcaga
    10801 cgcgattgat tctttaaatg catcaaatga ttcatctaaa atttggtcac tgtatgcttc
    10861 caatgtttgt ttttgataaa tttgaatagt tgcttttaag aaattcaaaa aatattgaaa
    10921 tacattaaag ttaaagtgat tatttgaaac aacaaactgg acattatctt ggtttgaaca
    10981 atttgagttg gcaaacgaat aaaccattgt ttgagttgta ttttcattat tgtttttgct
    11041 atttctgtta tttgtattta tactagttaa aaactcaact gaagtggaaa tatatttttc
    11101 ataaactttg gcacgttcgt ttaattttga aatcaatgaa tctcgtttta atatttcatg
    11161 tttgtaagaa tattcaaaag caggttgtgt gatttgattg gaaactgttg gtgttgtttc
    11221 taattgaata attcgagacc ttaaagattc aatttctttt tcaaggctat aattttttgt
    11281 attaagatca tttaattgag atttatgttc atttgaaatc tttaaagaat ttaaaaggtt
    11341 aaagtcttta aagttatttt taagagtttc taattctgca ttgatttgat cgtttttatc
    11401 ttttaaaact ctaccagcgg catacgatac ttgtaattct ttttcttttt ctgaaaaaca
    11461 attttcatat tcatttatat aattttggta g
//
