Basic Vector Information
pTT-PB-SOKMLN-Cer vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTT-PB-SOKMLN-Cer vector Sequence
LOCUS 40924_44399 13592 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pTT-PB-SOKMLN-Cer, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13592)
AUTHORS Debowski K, Warthemann R, Lentes J, Salinas-Riester G, Dressel R,
Langenstroth D, Gromoll J, Sasaki E, Behr R.
TITLE Non-Viral Generation of Marmoset Monkey iPS Cells by a
Six-Factor-in-One-Vector Approach
JOURNAL PLoS ONE 10 (3), E0118424 (2015)
PUBMED 25785453
REFERENCE 2 (bases 1 to 13592)
AUTHORS Debowski K.
TITLE Direct Submission
JOURNAL Submitted (11-AUG-2014) Stem Cell Biology Unit, German Primate
Center, Kellnerweg 4, Goettingen, Niedersachsen 37077, Germany
REFERENCE 3 (bases 1 to 13592)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 13592)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
date: "2015"; volume: "10"; issue: "3"; pages: "E0118424"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(11-AUG-2014) Stem Cell Biology Unit, German Primate Center,
Kellnerweg 4, Goettingen, Niedersachsen 37077, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..13592
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 4..318
/label=tight TRE promoter
/note="Tet-responsive promoter PTight, consisting of seven
tet operator sequences followed by the minimal CMV
promoter"
repeat_region 345..871
/label=piggyBac 5' terminal repeat
/note="piggyBac 5' terminal repeat"
enhancer 904..1283
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1286..1561
/label=chicken beta-actin promoter
intron 1562..2573
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
CDS 2696..3652
/label=mSox2
/note="Mus musculus transcription factor SOX-2 gene.
Belongs to the SRY-related HMG-box (SOX) family of
transcription factors, which is involved in the regulation
of embryonic development and in the determination of cell
fate"
CDS 3653..3718
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/label=F2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="VKQTLNFDLLKLAGDVESNPGP"
CDS 3725..4804
/label=hOct4
/note="Homo sapiens Oct-4 gene. Encodes a transcription
factor containing a POU homeodomain that plays a key role
in embryonic development and stem cell pluripotency.
Aberrant expression in adult tissues is associated with
tumorigenesis."
CDS 4805..4858
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 6269..6328
/codon_start=1
/product="2A peptide from equine rhinitis A virus
polyprotein"
/label=E2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="QCTNYALLKLAGDVESNPGP"
CDS 6338..7651
/gene="MYC"
/label=MYC
/note="Myc proto-oncogene protein from Callithrix jacchus.
Accession#: P49032"
CDS 7658..7711
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 7712..8338
/label=hLIN28A
/note="Human lin-28 homolog A gene. Encodes a LIN-28 family
RNA-binding protein that acts as a posttranscriptional
regulator of genes involved in development, self-renewal of
embryonic stem cells and metabolism. Overexpressed in human
embryonic stem cells, primary human tumors and human cancer
cell lines."
CDS 8345..8398
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
misc_feature 9340..9925
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 9938..10654
/label=mCerulean
/note="enhanced monomeric variant of CFP (Rizzo et al.,
2004)"
polyA_signal 10689..10913
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 10939..11388
/label=piggyBac 3' terminal repeat
/note="piggyBac 3' terminal repeat"
polyA_signal 11506..11587
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(11769..12357)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(12531..13388)
/label=AmpR
/note="beta-lactamase"
promoter complement(13389..13493)
/label=AmpR promoter
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