LOCUS       40924_44399       13592 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTT-PB-SOKMLN-Cer, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13592)
  AUTHORS   Debowski K, Warthemann R, Lentes J, Salinas-Riester G, Dressel R, 
            Langenstroth D, Gromoll J, Sasaki E, Behr R.
  TITLE     Non-Viral Generation of Marmoset Monkey iPS Cells by a 
            Six-Factor-in-One-Vector Approach
  JOURNAL   PLoS ONE 10 (3), E0118424 (2015)
  PUBMED    25785453
REFERENCE   2  (bases 1 to 13592)
  AUTHORS   Debowski K.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2014) Stem Cell Biology Unit, German Primate 
            Center, Kellnerweg 4, Goettingen, Niedersachsen 37077, Germany
REFERENCE   3  (bases 1 to 13592)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13592)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "3"; pages: "E0118424"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-AUG-2014) Stem Cell Biology Unit, German Primate Center, 
            Kellnerweg 4, Goettingen, Niedersachsen 37077, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..318
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     repeat_region   345..871
                     /label=piggyBac 5' terminal repeat
                     /note="piggyBac 5' terminal repeat"
     enhancer        904..1283
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1286..1561
                     /label=chicken beta-actin promoter
     intron          1562..2573
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             2696..3652
                     /label=mSox2
                     /note="Mus musculus transcription factor SOX-2 gene.
                     Belongs to the SRY-related HMG-box (SOX) family of 
                     transcription factors, which is involved in the regulation 
                     of embryonic development and in the determination of cell 
                     fate"
     CDS             3653..3718
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             3725..4804
                     /label=hOct4
                     /note="Homo sapiens Oct-4 gene. Encodes a transcription
                     factor containing a POU homeodomain that plays a key role 
                     in embryonic development and stem cell pluripotency. 
                     Aberrant expression in adult tissues is associated with 
                     tumorigenesis."
     CDS             4805..4858
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6269..6328
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             6338..7651
                     /gene="MYC"
                     /label=MYC
                     /note="Myc proto-oncogene protein from Callithrix jacchus. 
                     Accession#: P49032"
     CDS             7658..7711
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7712..8338
                     /label=hLIN28A
                     /note="Human lin-28 homolog A gene. Encodes a LIN-28 family
                     RNA-binding protein that acts as a posttranscriptional 
                     regulator of genes involved in development, self-renewal of
                     embryonic stem cells and metabolism. Overexpressed in human
                     embryonic stem cells, primary human tumors and human cancer
                     cell lines."
     CDS             8345..8398
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    9340..9925
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             9938..10654
                     /label=mCerulean
                     /note="enhanced monomeric variant of CFP (Rizzo et al.,
                     2004)"
     polyA_signal    10689..10913
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   10939..11388
                     /label=piggyBac 3' terminal repeat
                     /note="piggyBac 3' terminal repeat"
     polyA_signal    11506..11587
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(11769..12357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12531..13388)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(13389..13493)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
       61 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
      121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      181 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      241 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagaattaat tcgagctcgg tacctcgcgc gacttggttt
      361 gccattcttt agcgcgcgtc gcgtcacaca gcttggccac aatgtggttt ttgtcaaacg
      421 aagattctat gacgtgttta aagtttaggt cgagtaaagc gcaaatcttt tttaacccta
      481 gaaagatagt ctgcgtaaaa ttgacgcatg cattcttgaa atattgctct ctctttctaa
      541 atagcgcgaa tccgtcgctg tgcatttagg acatctcagt cgccgcttgg agctcccgtg
      601 aggcgtgctt gtcaatgcgg taagtgtcac tgattttgaa ctataacgac cgcgtgagtc
      661 aaaatgacgc atgattatct tttacgtgac ttttaagatt taactcatac gataattata
      721 ttgttatttc atgttctact tacgtgataa cttattatat atatattttc ttgttataga
      781 tatcgtgact aatatataat aaaatgggta gttctttaga cgatgagcat atcctctctg
      841 ctcttctgca aagcgatgac gagcttgttg gagatctcga tgtacgggcc agatatacgc
      901 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
      961 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1021 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1081 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
     1141 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     1201 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     1261 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
     1321 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     1381 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
     1441 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
     1501 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
     1561 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
     1621 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     1681 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
     1741 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
     1801 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
     1861 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1921 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1981 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     2041 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     2101 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     2161 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     2221 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     2281 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
     2341 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
     2401 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
     2461 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     2521 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     2581 gcaacgtgct ggttattgtg ctgtctcatc attttgggct agcgtttaaa cttaagcttg
     2641 gtaccgagct cggatccact agtccagtgt ggtggaattc gagctcggta ccaccatgta
     2701 caacatgatg gagacggagc tgaagccgcc gggcccgcag cagacttcgg ggggcggcgg
     2761 cggcaacccc accgcggcgg cggcggcggc gggtggcaac cagaagaaca gccccgaccg
     2821 cgtcaagcgg cccatgaacg ccttcatggt gtggtcccgc gggcagcggc gcaagatggc
     2881 ccaggagaac cccaagatgc acaactcgga gatcagcaag cgcctgggcg ccgagtggaa
     2941 gctgctgtcc gagacggaga agcggccgtt catcgacgag gccaagcggc tgcgcgcgct
     3001 gcacatgaag gagcacccgg attacaagta ccggccccgg cggaaaacca agacgctcat
     3061 gaagaaggat aagtacacgc tgcccggcgg gctgctggcg cccggcggca acggcatggc
     3121 cggcggggtc ggggtgggcg ccggcctggg cgcgggcgtg aaccagcgca tggacagcta
     3181 cgcgcacatg aacggctgga gcaacggcag ctacagcatg atgcaggagc agctgggcta
     3241 cccgcagcac ccgggcctca acgcgcacgg cgcggcgcag atgcagccca tgcaccgcta
     3301 cgacgtgagc gcgctgcagt acaactccat gaccagctcg cagacctaca tgaacggctc
     3361 gcccacctac agcatgtcct actcgcagca gggcacgccc ggcatggcgc tgggctccat
     3421 gggctcggtg gtcaagtcgg aggcgagctc cagcccgccg gtggtgacct cttcctcgca
     3481 ctccagggcg ccctgccagg ccggggacct ccgggacatg atcagcatgt acctccccgg
     3541 cgcggaggtg ccggagcccg ccgcgcccag cagactgcac atgtcccagc actaccagag
     3601 cggcccggtg cccggcacgg ccatcaacgg cacactgccg ctctcgcaca tggtgaaaca
     3661 gactttgaat tttgaccttc tcaagttggc gggagacgtg gagtccaacc cagggcccgg
     3721 atccatggcg ggacacctgg cttcggattt tgccttctcg ccccctccag gtggtggagg
     3781 tgatgggcca ggggggccgg agacaggctg ggttgatcct cggacctggc taagcttcca
     3841 gggccctcct ggagggccag gaatcgggcc aggggttggg ccaggcgctg aggtgtgggg
     3901 gattccccca tgccccccac cgtatgagtt ctgtgggggg atggcatact gtgggcctca
     3961 ggttggagtg gggctagtgc cccaaggtgg cttggagacc tctcagcctg agggcgaagc
     4021 aggagccggg gtggagagca attccgaggg ggcttccccg gagccctgca ccatcccccc
     4081 tggtgctgtg aagctggaga aggagaagct ggaacaaaac acggaggagt cccaagacat
     4141 caaagctctg cagaaagaac tcgagcaatt tgccaagctc ctgaagcaga agaggatcac
     4201 cctgggatat acacaggccg atgtggggct caccctgggg gttctctttg gaaaggtgtt
     4261 cagccaaacg accatctgcc gctttgaggc tctgcagctt agcttcaaga acatgtgtaa
     4321 gctgcggcct ttactgcaga agtgggtgga ggaagctgac aacaatgaaa atcttcagga
     4381 gatatgcaaa gcagaaaccc tcgtgcaggc ccggaagaga aagcgaacca gtatcgagaa
     4441 tcgagtgaga ggcagcctgg agaatttgtt cctgcagtgc ccgaaaccca cacttcagca
     4501 gatcagccac atcgcccagc agcttgggct cgagaaggat gtggtccgtg tgtggttctg
     4561 taaccggcgc cagaagggca agcgatcaag cagtgactat gcacaacgag aggattttga
     4621 ggctgctggg tcccctttct cagggggacc agtgtccttt cctctggccc cagggcccca
     4681 ttttggtacc ccaggctatg ggagccctca tttcactgca ctgtactcct cggtcccatt
     4741 ccctgagggg gaagccttcc cccctgtctc tgtccccact ctgggctctc ccatgcattc
     4801 caacgagggc agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccaac
     4861 gcgtatggct gtcagcgacg cgctgctccc atctttctcc acgttcgcgt ctggccccgc
     4921 aggaagggag aagacactgc gtcaagcagg tgctccaaat aaccgctggc gggaggagct
     4981 ctcccacatg aagcgacttc cccaggtgct tcctggccgc ccctacgacc tggcggcggc
     5041 taccgtggcc acagacctgg agagcggcgg agccggtgca gcttgcggtg gtagcaacct
     5101 ggcgccccta ccccggagag agacggagga gttcaacgat ctcctggact tggactttat
     5161 cctctccaat tcgctgtccc atccagagtc ggtggccgcc accgtgtcct cgtcagcatc
     5221 agcgtcttcc tcgtcgtccc cgacgagcag cggccctgcc agcgcgccct ccacctgcag
     5281 cttcagctac ccgatccggg ccgggagcga cccaggcgtg gcgccgggca gcacgggtgg
     5341 aggcctcctc tatggcaggg agtccgctcc ccctccgaca gctcccttca acctggctga
     5401 catcaacgac gtgagcccct cgggcggctt cgtggccgag ctcctgcggc cggagttgga
     5461 cccggtgtac attcccccgc agcagccgca gccgccaggt ggcgggctga tgggcaagtt
     5521 cgtgctgaag gcgtctctgc cccctggcag cgagtacggc agcccgtcgg tcatcagcgt
     5581 cagcaaaggc agccctgacg gcagccaccc ggtggtggtg gcgccctaca acggcgggcc
     5641 gccgcgcatg tgccccaaga tcaagcagga ggcggtctcc tcgtgcaccc acttgggagc
     5701 tggaccccct ctcagcaatg gccaccggcc ggctgcacac gacttccccc tggggcggca
     5761 gctccccagc aggactaccc cgaccctggg tcctgaggaa ctgctgagca gcagggactg
     5821 tcaccctgcc ctgccacttc cccccggctt ccatccccac ccggggccca attacccatc
     5881 cttcctgcct gaccagatgc agccgcaagt cccgccgctc cattaccaag agctcatgcc
     5941 acccggttcc tgcatgccgg aggagcctaa gccaaagagg ggaagacgat cttggccccg
     6001 gaagaggacc gccacccaca cttgtgatta tgcgggctgc ggcaaaacct acacaaagag
     6061 ttcccatctc aaggcacacc tgcgaaccca cacaggtgag aaaccttacc actgtgactg
     6121 ggacggctgt ggatggaaat tcgcccgctc agatgaactg accaggcact accgtaaaca
     6181 cactgggcac cgcccattcc agtgccagaa atgcgaccga gcattttcca ggtcggacca
     6241 cctcgcctta cacatgaaga ggcattttca atgtactaac tacgctttgt tgaaactcgc
     6301 tggcgatgtt gaaagtaacc ccggtcctgc ggccgcaatg cccctcaacg tcagcttcag
     6361 caacaggaac tatgacctcg actacgactc ggtgcagccg tatttctact gcgacgagga
     6421 agagaacttc taccagcagc agcagcagag cgagctgcag ccgccagcgc ccagcgagga
     6481 tatctggaag aaattcgagc tgctgcccac cccgcctctg tccccgagcc gccgctcggg
     6541 gctctgctcg acctcctgcg tttcagtcac gcccttctcc cctcgtggag acaacgacgg
     6601 cggtggcggg agcttctcca ctgccgacca gctggagatg gtgactgagc tgctgggagg
     6661 agacatggtg aaccagagct tcatctgcga cccggacgac gagaccttca tcaaaaacat
     6721 catcatccag gactgtatgt ggagcggctt ttcggccgcc gccaagctcg tctcagagaa
     6781 gctggcctcc taccaggctg cgcgcaaaga cagcagcagc ccgaaccccg cccgcgggca
     6841 cagcgtctgc tccacctcca gcttgtacct gcaggatctg agcgccgccg cctcggagtg
     6901 catcgacccc tcggtggtat tcccgtaccc gctcaatgac agcagctcgc ccaagccctg
     6961 cgcctcgcca gactccagcg ccttctccac gtcctcggac tctctgctct cctcgacgga
     7021 gtcctccccg cgggccagcc ccgagcccct ggtgctccat gaagagacac cgcccactac
     7081 cagcagcgac tctgaggagg aacaagagga tgaagaaatc gatgtcgttt ctgtggaaaa
     7141 gaggcagcct cctggcaaaa ggtcagagtc aggatcaccc tcttctggag gccacagcaa
     7201 acctcctcac agcccactgg tcctcaagag gtgccacgtc tccacacatc agcacaatta
     7261 cgcagcccct ccctccacgc ggaaggacta cccagctgcc aagagggtca agttggacag
     7321 tgtcagagtc ctgaggcaga tcagcaacaa ccgaaaatgc accagcccca ggtcctcgga
     7381 caccgaagag aatgacaaga ggcgaacgca caacgtcctg gaacgccaga ggaggaacga
     7441 gctcaaacgg agcttctttg ccctgcgtga ccaaatccca gagctggaaa acaatgaaaa
     7501 ggcccccaag gtagttattc ttaagaaagc cacaacatac atcctgtccg tccaagcaga
     7561 ggagcaaaag ctcatttctg aaaaggactt gttgcggaag cgacgagaac agttgaaaca
     7621 caaacttgaa cagctgcgga actcttgtgc aaccggtgag ggcagaggaa gtctgctaac
     7681 atgcggtgac gtcgaggaga atcctggccc aatgggctcc gtgtccaacc aacagtttgc
     7741 aggtggctgc gccaaggcgg cagaagaggc gccggaggag gcgccggagg atgcggcccg
     7801 ggcggcggac gagcctcagc tgctgcacgg tgcaggcatc tgcaagtggt tcaacgtgcg
     7861 catggggttc ggcttcctgt ccatgaccgc ccgcgccggg gtcgcgctcg accccccagt
     7921 ggacgtcttt gtgcaccaga gtaagctgca catggagggc ttccggagct tgaaggaagg
     7981 tgaggccgtg gagttcacct ttaagaagtc agccaagggt ctggaatcca tccgtgtcac
     8041 tggacctggt ggggtgttct gtattgggag tgagaggcgg ccaaagggga agaacatgca
     8101 gaagcgcaga tcaaaaggag acaggtgcta caactgtgga ggtctagacc atcatgccaa
     8161 ggaatgcaag ctgccacccc agcccaagaa gtgccacttc tgccagagca tcagccatat
     8221 ggtagcctca tgtccactga aggcccagca ggcccctagc gcacagggaa agccaacata
     8281 ctttcgggag gaagaagaag aaatccacag ccctaccctg ctcccggagg cccagaattt
     8341 cgaagagggc agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccaat
     8401 gagtatggat ccagtttgtt cacaaagcct gccttgctcc gaagcatccg actgtggaga
     8461 atcttcacct acgcctgtga tttgtgagcc tgaagaaaat tatccatcct tgcaaatgtg
     8521 ttctgctgag atgcctcaca cagagactgt ctcccctctt ccttcctcca tggatctgct
     8581 tactcaggac agccctgatt cttccaccag tcccaaaggg aaacgaccca ctgctgcaga
     8641 gagtagccct gcagtgaagg aagacaaggt cccggtcaag aaacagaaga ccagaactgt
     8701 gttctcttcc acccagctgt gtgtactcaa tgatagattt cagagacaga aatatctcag
     8761 tcttcagcag atgcaagaac tttccaacat cctgaatctt agctacaaac aggtgaagac
     8821 ctggttccag aaccagagaa tgaaatctaa gaggtggcag aaaaacaact ggccaaggaa
     8881 caccagtgat gtgactcaga aggcctcagc acccacctac cccagcctct actcttccta
     8941 ccaccaggga tgcctggtga acacagctgg gaaccttccg gtatggaaca accagacctg
     9001 gagcaattca acctggagca accagacccg gaacatccag tcctggagca accactcctg
     9061 gaacactcag acctggtgca cccagtcctg gaacactcag gcctggagca gtcccttcta
     9121 taactgtgga gaggaacctc tgcagtcctg tatgcagttt cagccaaatt ctcctgccag
     9181 tgacttggag gctgccttgg aaactgctgg ggaaaaccat aatgtaatac agcagaccac
     9241 taggtatttg aatacttcac aaaccatgga tttattccta aactactcta cgaacatgcc
     9301 acctgaagat gtgtgaggcg cgcctaaata aataagtcga cctctccctc ccccccccct
     9361 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     9421 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     9481 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     9541 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     9601 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     9661 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     9721 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     9781 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     9841 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     9901 acacgatgat aagcttgcca caaccctcta gaccaccatg gtgagcaagg gcgaggagct
     9961 gttcaccggg gtagtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
    10021 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
    10081 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg
    10141 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
    10201 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
    10261 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
    10321 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca acgccatcag
    10381 cgacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
    10441 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
    10501 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
    10561 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
    10621 cgggatcact ctcggcatgg acgagctgta caagtaaggg cccgtttaaa cccgctgatc
    10681 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
    10741 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
    10801 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
    10861 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
    10921 ggcggaaaga accagctgcg agagcataat attgatatgt gccaaagttg tttctgactg
    10981 actaataagt ataatttgtt tctattatgt ataagttaag ctaattactt attttataat
    11041 acaacatgac tgtttttaaa gtacaaaata agtttatttt tgtaaaagag agaatgttta
    11101 aaagttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa taaataaata
    11161 aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat ataataaaac
    11221 ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa acacatgcgt
    11281 caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt ctagggttaa
    11341 ataatagttt ctaatttttt tattattcag cctgctgtcg tgaatacctc tagctagagg
    11401 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
    11461 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
    11521 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    11581 tcactgcctc gagcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    11641 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    11701 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    11761 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    11821 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    11881 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    11941 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
    12001 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    12061 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    12121 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    12181 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
    12241 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    12301 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    12361 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    12421 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    12481 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    12541 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    12601 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    12661 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    12721 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    12781 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    12841 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    12901 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    12961 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    13021 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    13081 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    13141 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    13201 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    13261 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    13321 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    13381 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    13441 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    13501 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
    13561 aataggcgta tcacgaggcc ctttcgtctt ca
//
