Basic Vector Information
- Vector Name:
- pTScDisp
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5483 bp
- Type:
- Phage display vector
- Replication origin:
- ori
- Source/Author:
- Kolesnikov AV, Kozyr AV, Ignatova AN, Zakharova MY.
pTScDisp vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTScDisp vector Sequence
LOCUS 40924_44389 5483 bp DNA circular SYN 18-DEC-2018
DEFINITION Phage display vector pTScDisp, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5483)
AUTHORS Kolesnikov AV, Kozyr AV, Ignatova AN, Zakharova MY.
TITLE Improved purification of filamentous bacteriophages for phage
display using size-exclusion chromatography
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5483)
AUTHORS Kolesnikov AV, Kozyr AV, Ignatova AN, Zakharova MY.
TITLE Direct Submission
JOURNAL Submitted (06-MAY-2004) Laboratory of Biocatalysis, Shemyakin and
Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya
16/10, Moscow 117997, Russia
REFERENCE 3 (bases 1 to 5483)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5483)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(06-MAY-2004) Laboratory of Biocatalysis, Shemyakin and Ovchinnikov
Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, Moscow
117997, Russia"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5483
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind complement(108..129)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(145..1224)
/label=lacI
/note="lac repressor"
promoter complement(1225..1302)
/label=lacI promoter
protein_bind 1472..1488
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 1539..1561
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
misc_feature 1572..1631
/label=pelB leader for periplasmic migration
/note="pelB leader for periplasmic migration"
misc_feature 1679..1726
/label=ScFv linker
/note="ScFv linker"
misc_feature 1787..2326
/gene="pIII"
/note="m13 minor coat protein fragment; this fragment of
the protein is used for N-terminal fusion expression during
phage display"
gene 1787..2326
/gene="pIII"
/label=pIII
terminator 2538..2624
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2716..2743
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
rep_origin 2841..3296
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3578..3682
/label=AmpR promoter
CDS 3683..4540
/label=AmpR
/note="beta-lactamase"
rep_origin 4714..5302
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
rep_origin 5305..5311
/label=ColE1 ori
/note="ColE1 ori"
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