LOCUS       40924_44389        5483 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Phage display vector pTScDisp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Kolesnikov AV, Kozyr AV, Ignatova AN, Zakharova MY.
  TITLE     Improved purification of filamentous bacteriophages for phage 
            display using size-exclusion chromatography
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Kolesnikov AV, Kozyr AV, Ignatova AN, Zakharova MY.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2004) Laboratory of Biocatalysis, Shemyakin and 
            Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 
            16/10, Moscow 117997, Russia
REFERENCE   3  (bases 1 to 5483)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5483)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-2004) Laboratory of Biocatalysis, Shemyakin and Ovchinnikov 
            Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, Moscow 
            117997, Russia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(108..129)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(145..1224)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(1225..1302)
                     /label=lacI promoter
     protein_bind    1472..1488
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1539..1561
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    1572..1631
                     /label=pelB leader for periplasmic migration
                     /note="pelB leader for periplasmic migration"
     misc_feature    1679..1726
                     /label=ScFv linker
                     /note="ScFv linker"
     misc_feature    1787..2326
                     /gene="pIII"
                     /note="m13 minor coat protein fragment; this fragment of
                     the protein is used for N-terminal fusion expression during
                     phage display"
     gene            1787..2326
                     /gene="pIII"
                     /label=pIII
     terminator      2538..2624
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2716..2743
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      2841..3296
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3578..3682
                     /label=AmpR promoter
     CDS             3683..4540
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4714..5302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5305..5311
                     /label=ColE1 ori
                     /note="ColE1 ori"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgga
       61 ctgggttgaa ggctctcaag ggcagtcggt cgagatcccg gtgcctaatg agtgagctaa
      121 cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt
      241 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
      301 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
      361 ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga
      421 gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat
      481 ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt
      541 ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg
      601 attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa
      661 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
      721 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc
      781 aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc
      841 cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc
      901 tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg
      961 atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga
     1021 ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg
     1081 aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg
     1141 gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac
     1201 atcgtataac gttactggtt tcacattcac catcctgaat tgactctctt ccgggcgcta
     1261 tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc
     1321 ccttatgcga ctcctgcatt aggccatgga ctccccatcc ccctgttgac aattaatcat
     1381 cggctcgtat aaaatacgct gaggacgtaa tccggtacct gaggggtagg gggacaactg
     1441 ttaattagta gccgagcata tatgtgtgga attgtgagcg gataacaatt tcacacagga
     1501 aacagaattg atccataact aactaatcta gaaataattt tgtttaactt taagaaggag
     1561 atatacatat gaaatacctg tccctgccga ccgctgctgc tggtctgctg ctcctcgcgg
     1621 cccagccggc catggcccag gtccaactgc aggagctcga gatcaaacgg gcggccgccc
     1681 gaacggtgct tctaaatccg gttcttccca gaccggctct gctccgacta gtggatcccc
     1741 cgggctgcag gaattcgata tcaagcttga aatccagggc gcgccgccgg ttaattctgg
     1801 cggctctgag ggtggcggtt ctgagggtgg cggctctgag ggtggcggtt ccggtggtgg
     1861 ctctggttcc ggtgattttg attatgaaaa gatggcaaac gctaataagg gggctatgac
     1921 cgaaaatgcc gatgaaaacg cgctacagtc tgacgctaaa ggcaaacttg attctgtcgc
     1981 tactgattac ggtgctgcta tcgacggttt cattggtgac gtttccggcc ttgctaatgg
     2041 taatggtgct actggtgatt ttgctggctc taattcccaa atggctcaag tcggtgacgg
     2101 tgataattca cctttaatga ataatttccg tcaatattta ccttccctcc ctcaatcggt
     2161 tgaatgtcgc ccttttgtct ttggcgctgg taaaccatat gaattttcta ttgattgtga
     2221 caaaataaac ttattccgtg gtgtctttgc gtttctttta tatgttgcca cctttatgta
     2281 tgtattttcg acgtttgcta acatactgcg taataaggag tcttaataag aattcggctg
     2341 ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg
     2401 tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc
     2461 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt
     2521 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2581 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
     2641 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
     2701 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc
     2761 ttaagcttcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     2821 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2881 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     2941 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     3001 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     3061 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3121 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3181 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3241 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     3301 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     3361 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     3421 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     3481 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     3541 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3601 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3661 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3721 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3781 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3841 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     3901 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     3961 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4021 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4081 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4141 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4201 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4261 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4321 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4381 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4441 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4501 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4561 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4621 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4681 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4741 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4801 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4861 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4921 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4981 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5041 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5101 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5161 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5221 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5281 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5341 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5401 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5461 gtcagtgagc gaggaagcgg aag
//
