pTRXFUS vector (V002519)

LOCUS       thioredoxin_gene        3585 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fusion cloning vector pTRXFUS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    thioredoxin gene fusion vector
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   LaVallie ER, DiBlasio EA, Kovacic S, Grant KL, Schendel PF, McCoy 
            JM.
  TITLE     A thioredoxin gene fusion expression system that circumvents 
            inclusion body formation in the E. coli cytoplasm
  JOURNAL   Biotechnology (N.Y.) 11 (2), 187-193 (1993)
  PUBMED    7763371
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   LaVallie ER.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-1994) Edward R. LaVallie, Genetics Institute, 87 
            CambridgePark Drive, Cambridge, MA 02140, USA
REFERENCE   3  (bases 1 to 3585)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3585)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechnology (N.Y.)"; date: "1993"; volume: "11"; issue: "2"; 
            pages: "187-193"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-NOV-1994) Edward R. LaVallie, Genetics Institute, 87 
            CambridgePark Drive, Cambridge, MA 02140, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      2159..2187
                     /label=lambda pL promoter
                     /note="lambda pL promoter"
                     /regulatory_class="promoter"
     CDS             2416..2481
                     /label=lambda N peptide
                     /note="N-terminal RNA-binding domain from the lambda
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
     CDS             2724..3050
                     /label=TrxA
                     /note="E. coli thioredoxin"
     CDS             3066..3080
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    3081..3112
                     /label=polylinker cloning site
                     /note="polylinker cloning site"
     regulatory      3113..3179
                     /label=aspA transcription terminator
                     /note="aspA transcription terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(3189..3205)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"