pTRXFUS vector (V002519)

MSDS

Basic Vector Information

Vector Name:: pTRXFUS

Antibiotic Resistance:: Ampicillin

Length:: 3585 bp

Type:: Fusion cloning vector

Replication origin:: ori

Source/Author:: LaVallie ER, DiBlasio EA, Kovacic S, Grant KL, Schendel PF, McCoy JM.

pTRXFUS vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTRXFUS vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       thioredoxin_gene        3585 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fusion cloning vector pTRXFUS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    thioredoxin gene fusion vector.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   LaVallie ER, DiBlasio EA, Kovacic S, Grant KL, Schendel PF, McCoy 
            JM.
  TITLE     A thioredoxin gene fusion expression system that circumvents 
            inclusion body formation in the E. coli cytoplasm
  JOURNAL   Biotechnology (N.Y.) 11 (2), 187-193 (1993)
  PUBMED    7763371
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   LaVallie ER.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-1994) Edward R. LaVallie, Genetics Institute, 87 
            CambridgePark Drive, Cambridge, MA 02140, USA
REFERENCE   3  (bases 1 to 3585)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3585)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechnology (N.Y.)"; date: "1993"; volume: "11"; issue: "2"; 
            pages: "187-193"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-NOV-1994) Edward R. LaVallie, Genetics Institute, 87 
            CambridgePark Drive, Cambridge, MA 02140, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      2159..2187
                     /label=lambda pL promoter
                     /note="lambda pL promoter"
                     /regulatory_class="promoter"
     CDS             2416..2481
                     /label=lambda N peptide
                     /note="N-terminal RNA-binding domain from the lambda
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
     CDS             2724..3050
                     /label=TrxA
                     /note="E. coli thioredoxin"
     CDS             3066..3080
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    3081..3112
                     /label=polylinker cloning site
                     /note="polylinker cloning site"
     regulatory      3113..3179
                     /label=aspA transcription terminator
                     /note="aspA transcription terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(3189..3205)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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