LOCUS       thioredoxin_gene        3585 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fusion cloning vector pTRXFUS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    thioredoxin gene fusion vector.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   LaVallie ER, DiBlasio EA, Kovacic S, Grant KL, Schendel PF, McCoy 
            JM.
  TITLE     A thioredoxin gene fusion expression system that circumvents 
            inclusion body formation in the E. coli cytoplasm
  JOURNAL   Biotechnology (N.Y.) 11 (2), 187-193 (1993)
  PUBMED    7763371
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   LaVallie ER.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-1994) Edward R. LaVallie, Genetics Institute, 87 
            CambridgePark Drive, Cambridge, MA 02140, USA
REFERENCE   3  (bases 1 to 3585)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3585)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechnology (N.Y.)"; date: "1993"; volume: "11"; issue: "2"; 
            pages: "187-193"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-NOV-1994) Edward R. LaVallie, Genetics Institute, 87 
            CambridgePark Drive, Cambridge, MA 02140, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      2159..2187
                     /label=lambda pL promoter
                     /note="lambda pL promoter"
                     /regulatory_class="promoter"
     CDS             2416..2481
                     /label=lambda N peptide
                     /note="N-terminal RNA-binding domain from the lambda
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
     CDS             2724..3050
                     /label=TrxA
                     /note="E. coli thioredoxin"
     CDS             3066..3080
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    3081..3112
                     /label=polylinker cloning site
                     /note="polylinker cloning site"
     regulatory      3113..3179
                     /label=aspA transcription terminator
                     /note="aspA transcription terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(3189..3205)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagaatt gatctctcac ctaccaaaca atgcccccct gcaaaaaata
     2101 aattcatata aaaaacatac agataaccat ctgcggtgat aaattatctc tggcggtgtt
     2161 gacataaata ccactggcgg tgatactgag cacatcagca ggacgcactg accaccatga
     2221 aggtgacgct cttaaaaatt aagccctgaa gaagggcagc attcaaagca gaaggctttg
     2281 gggtgtgtga tacgaaacga agcattggcc gtaagtgcga ttccggatta gctgccaatg
     2341 tgccaatcgc ggggggtttt cgttcaggac tacaactgcc acacaccacc aaagctaact
     2401 gacaggagaa tccagatgga tgcacaaaca cgccgccgcg aacgtcgcgc agagaaacag
     2461 gctcaatgga aagcagcaaa tcccctgttg gttggggtaa gcgcaaaacc agttccgaaa
     2521 gattttttta actataaacg ctgatggaag cgtttatgcg gaagaggtaa agcccttccc
     2581 gagtaacaaa aaaacaacag cataaataac cccgctctta cacattccag ccctgaaaaa
     2641 gggcatcaaa ttaaaccaca cctatggtgt atgcatttat ttgcatacat tcaatcaatt
     2701 gttatctaag gaaatactta catatgagcg ataaaattat tcacctgact gacgacagtt
     2761 ttgacacgga tgtactcaaa gcggacgggg cgatcctcgt cgatttctgg gcagagtggt
     2821 gcggtccgtg caaaatgatc gccccgattc tggatgaaat cgctgacgaa tatcagggca
     2881 aactgaccgt tgcaaaactg aacatcgatc aaaaccctgg cactgcgccg aaatatggca
     2941 tccgtggtat cccgactctg ctgctgttca aaaacggtga agtggcggca accaaagtgg
     3001 gtgcactgtc taaaggtcag ttgaaagagt tcctcgacgc taacctggcc ggttctggtt
     3061 ctggtgatga cgatgacaag gtacccgggg atcctctaga gtcgacctgc agtaatcgta
     3121 cagggtagta caaataaaaa aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     3181 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     3241 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     3301 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     3361 tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa
     3421 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     3481 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     3541 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcga
//
