pTriplEx2 vector (V002526)

Basic Vector Information

      • Vector Name:
      • pTriplEx2
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 3589 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Kalnine N.

pTriplEx2 vector Vector Map

pTriplEx23589 bp6001200180024003000CAP binding sitelac promoterlac operatorM13 revSP6 promoter5'UTRM13 revT7 promoterM13 fwdf1 oriloxPAmpR promoterAmpRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTriplEx2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_44257        3589 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTriplEx2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Kalnine N.
  TITLE     pTriplEx2 - cDNA library construction vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Kalnine N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2007) Informatics, Clontech, 1290 Terra Bella 
            Ave., Mountain View, CA 94043, USA
REFERENCE   3  (bases 1 to 3589)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3589)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-AUG-2007) Informatics, Clontech, 1290 Terra Bella Ave., Mountain
            View, CA 94043, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        238..256
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5'UTR           276..413
                     /label=derived from Escherichia coli ompA
                     /note="derived from Escherichia coli ompA"
     primer_bind     457..473
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(662..680)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(687..703)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(844..1299)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     protein_bind    complement(1359..1392)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1683..1787
                     /label=AmpR promoter
     CDS             1788..2645
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2819..3407
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

This page is informational only.