Basic Vector Information
pTriplEx2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTriplEx2 vector Sequence
LOCUS 40924_44257 3589 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pTriplEx2, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3589) AUTHORS Kalnine N. TITLE pTriplEx2 - cDNA library construction vector JOURNAL Unpublished REFERENCE 2 (bases 1 to 3589) AUTHORS Kalnine N. TITLE Direct Submission JOURNAL Submitted (29-AUG-2007) Informatics, Clontech, 1290 Terra Bella Ave., Mountain View, CA 94043, USA REFERENCE 3 (bases 1 to 3589) TITLE Direct Submission REFERENCE 4 (bases 1 to 3589) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (29-AUG-2007) Informatics, Clontech, 1290 Terra Bella Ave., Mountain View, CA 94043, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..3589 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 106..127 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 142..172 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 180..196 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 204..220 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 238..256 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" 5'UTR 276..413 /label=derived from Escherichia coli ompA /note="derived from Escherichia coli ompA" primer_bind 457..473 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter complement(662..680) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(687..703) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin complement(844..1299) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" protein_bind complement(1359..1392) /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." promoter 1683..1787 /label=AmpR promoter CDS 1788..2645 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 2819..3407 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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