LOCUS       40924_44257        3589 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTriplEx2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Kalnine N.
  TITLE     pTriplEx2 - cDNA library construction vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Kalnine N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2007) Informatics, Clontech, 1290 Terra Bella 
            Ave., Mountain View, CA 94043, USA
REFERENCE   3  (bases 1 to 3589)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3589)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-AUG-2007) Informatics, Clontech, 1290 Terra Bella Ave., Mountain
            View, CA 94043, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        238..256
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5'UTR           276..413
                     /label=derived from Escherichia coli ompA
                     /note="derived from Escherichia coli ompA"
     primer_bind     457..473
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(662..680)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(687..703)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(844..1299)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     protein_bind    complement(1359..1392)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1683..1787
                     /label=AmpR promoter
     CDS             1788..2645
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2819..3407
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctatt
      241 taggtgacac tatagaatac tcaagctagc tctagcttta catcgcaagg gtgtcggcat
      301 aagccgaaga tatcggtaga gttatattga gcagatcccc cggtgaagga tttaaccgtg
      361 ttatctcgtt ggaatattca tggcgtattt tggatgataa cgaggcgcaa aaaatgaaaa
      421 gtactctagc aattgtgagc ggacaacaat ttcacacagg aaacagctat gaccatgatt
      481 acgccaagct ccgagatctg gacgagcttt tttttttttt ctcgggaagc gcgccattgt
      541 gttggtaccc gggaattcgg ccattatggc ctgcaggatc cggccgcctc ggcccagtcg
      601 actctagact cgagcaagct tatgcatgcg gccgcaattc gagctcactt ggccaattcg
      661 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
      721 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      781 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      841 tggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatatttgt taaatcagct
      901 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
      961 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     1021 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     1081 ccaaatcaag ttttttgcgg tcgaggtgcc gtaaagctct aaatcggaac cctaaaggga
     1141 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     1201 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     1261 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcctgatgcg gtattttctc
     1321 cttacgcatc tgtgcggtat ttcacaccgc atagatcgat aacttcgtat agcatacatt
     1381 atacgaagtt atttaaatcg atatggtgca ctctcagtac aatctgctct gatgccgcat
     1441 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     1501 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     1561 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     1621 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     1681 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1741 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     1801 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     1861 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     1921 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     1981 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     2041 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2101 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     2161 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     2221 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     2281 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     2341 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     2401 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     2461 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     2521 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     2581 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2641 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2701 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2761 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2821 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2881 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2941 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     3001 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3061 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3121 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3181 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3241 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3301 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3361 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3421 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3481 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3541 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaaga
//
