Basic Vector Information
| Basic Vector Information | |||
|---|---|---|---|
| Vector Name | pTrc-TrxTAL | Antibiotic Resistance | Ampicillin |
| Length | 6022 bp | Type | Cloning vector |
| Source | Kim B, Binkley R, Kim HU, Lee SY. | ||
pTrc-TrxTAL vector Vector Map
Plasmid Resuspension protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTrc-TrxTAL vector Sequence
LOCUS Exported 6022 bp ds-DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pTrc-TrxTAL, complete sequence.
ACCESSION MH488931
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6022)
AUTHORS Kim B, Binkley R, Kim HU, Lee SY.
TITLE Metabolic engineering of Escherichia coli for the enhanced
production of l-tyrosine
JOURNAL Biotechnol. Bioeng. (2018) In press
PUBMED 30019750
REFERENCE 2 (bases 1 to 6022)
AUTHORS Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
TITLE Direct Submission
JOURNAL Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
Korea Advanced Institute of Science and Technology, Daehak-ro 291,
Daejeon 34141, South Korea
REFERENCE 3 (bases 1 to 6022)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6022
/organism="Cloning vector pTrc-TrxTAL"
/lab_host="Escherichia coli"
/mol_type="other DNA"
/db_xref="taxon:2301175"
regulatory 193..246
/regulatory_class="promoter"
/note="Trc promoter"
promoter 193..222
/label=trc promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 230..246
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
5'UTR 247..271
/gene="TAL"
gene 247..271
/gene="TAL"
/label=TAL
misc_feature 272..598
/gene="trxA"
/note="similar to thioredoxin; derived from Escherichia
coli; N-terminal thioredoxin-tag"
gene 272..598
/gene="trxA"
/label=trxA
CDS 272..595
/codon_start=1
/gene="trxA"
/product="E. coli thioredoxin"
/label=TrxA
/translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
DANL"
misc_feature 599..2128
/gene="TAL"
/note="similar to tyrosine ammonia-lyase; derived from
Saccharothrix espanaensis"
gene 599..2128
/gene="TAL"
/label=TAL
regulatory 2255..2580
/regulatory_class="terminator"
/note="rrnBT1T2"
terminator 2375..2461
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2553..2580
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 2600..2691
/gene="bla"
/label=AmpR promoter
CDS 2692..3552
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=bla
/protein_id="AXN70022.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
gene 2692..3552
/gene="bla"
/label=bla
rep_origin 3707..4326
/label=pBR322
/note="pBR322"
rep_origin 3723..4311
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 4497..4637
/label=bom
/note="basis of mobility region from pBR322"
promoter 4823..4900
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 4892..5983
/codon_start=1
/gene="lacIq"
/product="lac repressor protein"
/label=lacIq
/protein_id="AXN70023.1"
/translation="MVNVKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAM
AELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVER
SGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGD
WSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTED
SSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTA
SPRALADSLMQLARQVSRLESGQ"
gene 4892..5983
/gene="lacIq"
/label=lacIq
CDS 4901..5983
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
This page is informational only.