LOCUS       40924_43923        6022 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTrc-TrxTAL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 6022)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6022)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     5'UTR           247..271
                     /gene="TAL"
     gene            247..271
                     /gene="TAL"
                     /label=TAL
     CDS             272..598
                     /label=TrxA
                     /note="E. coli thioredoxin"
     misc_feature    599..2128
                     /gene="TAL"
                     /note="similar to tyrosine ammonia-lyase; derived from 
                     Saccharothrix espanaensis"
     gene            599..2128
                     /gene="TAL"
                     /label=TAL
     terminator      2375..2461
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2553..2580
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2600..2691
                     /label=AmpR promoter
     CDS             2692..3549
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3723..4311
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4497..4637)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        4823..4900
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4901..5980
                     /label=lacI
                     /note="lac repressor"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaaagac cgaggaaaag gagcatcgca aatgagcgat aaaattattc acctgactga
      301 cgacagtttt gacacggatg tactcaaagc ggacggggcg atcctcgtcg atttctgggc
      361 agagtggtgc ggtccgtgca aaatgatcgc cccgattctg gatgaaatcg ctgacgaata
      421 tcagggcaaa ctgaccgttg caaaactgaa catcgatcaa aaccctggca ctgcgccgaa
      481 atatggcatc cgtggtatcc cgactctgct gctgttcaaa aacggtgaag tggcggcaac
      541 caaagtgggt gcactgtcta aaggtcagtt gaaagagttc ctcgacgcta acctggcgac
      601 gcaggtcgtg gaacgtcagg ctgatcggct cagcagcagg gagtacctgg cccgggtcgt
      661 gcgcagcgcc gggtgggacg ccggtctcac ctcgtgcacc gacgaggaga tcgtccggat
      721 gggcgcgagc gcgcgcacca tcgaggagta cctgaagtcc gacaagccca tctacggcct
      781 gacgcagggc ttcggtccgc tggtgctgtt cgacgccgac tcggagctgg agcagggcgg
      841 ctcgctgatc tcgcacctgg gcaccggcca gggcgcgcca ctggccccgg aggtgtcgcg
      901 gctgatcctc tggctgcgca tccagaacat gcgcaagggg tactcggcgg tctcgccggt
      961 gttctggcag aagctcgccg acctgtggaa caaggggttc accccggcga tcccccggca
     1021 cggcacggtc agcgcgagcg gcgacctgca accgctggcg cacgccgcgc tcgccttcac
     1081 cggtgtcggc gaggcgtgga cccgggacgc cgacggccgg tggtccaccg tgccggccgt
     1141 ggacgcgctc gccgcgctgg gggcggagcc gttcgactgg ccggtgcgcg aggcgctggc
     1201 gttcgtcaac gggaccggcg cgagcctcgc ggtggctgtg ctcaaccacc ggtccgccct
     1261 gcggctggtc cgcgcctgcg ccgtgctctc cgcgcggctg gcgaccctgc tgggggccaa
     1321 tcccgagcac tacgacgtgg ggcacggtgt cgcgcgcggc caggtcggtc agctgaccgc
     1381 ggcggagtgg atccggcagg ggctgccccg gggcatggtg cgcgacggca gccgcccgct
     1441 ccaggagccg tacagcctgc ggtgcgcgcc gcaggtgctc ggcgcggtgc tcgaccagct
     1501 cgacggcgcg ggcgacgtgc tggcgcggga ggtcgacggc tgccaggaca acccgatcac
     1561 ctacgagggc gagctgctgc acggcggcaa cttccacgcc atgccggtgg gtttcgcctc
     1621 cgaccagatc gggttggcca tgcacatggc cgcctacctg gccgagcgcc agctgggtct
     1681 gctggtcagc ccggtgacca acggcgacct gccgcccatg ctcaccccgc gcgccgggcg
     1741 cggtgccggg ctggccgggg tgcagatcag cgcgacctcg ttcgtctcgc ggatccggca
     1801 gctggtgttc cccgcctcgc tgaccaccct gccgaccaac ggctggaacc aggaccacgt
     1861 gccgatggcg ctcaacgggg cgaactcggt gttcgaggcg ttggagctcg gctggctgac
     1921 ggtcgggtcg ctggcggtgg gcgtcgcgca gctcgcggcc atgaccggcc acgccgcgga
     1981 gggcgtctgg gcggagctgg ccgggatctg cccgccgctg gacgccgacc gcccgctggg
     2041 cgccgaggtg cgcgccgcgc gcgacctgct gtccgcgcac gcggaccaac tgctcgtcga
     2101 cgaggcagac gggaaggatt tcggatgagt acccggggat cctctagagt cgacctgcag
     2161 gcatgcaagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa
     2221 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
     2281 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
     2341 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
     2401 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     2461 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
     2521 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
     2581 tgcgtttcta caaactcttt ttgtttattt ttctaaatac attcaaatat gtatccgctc
     2641 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     2701 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2761 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2821 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     2881 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     2941 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     3001 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     3061 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     3121 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     3181 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctacagca
     3241 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     3301 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     3361 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     3421 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     3481 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3541 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3601 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3661 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3721 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3781 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3841 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     3901 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3961 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4021 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4081 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     4141 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4201 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4261 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4321 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4381 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4441 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     4501 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     4561 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     4621 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4681 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4741 agaggttttc accgtcatca ccgaaacgcg cgaggcagca gatcaattcg cgcgcgaagg
     4801 cgaagcggca tgcatttacg ttgacaccat cgaatggtgc aaaacctttc gcggtatggc
     4861 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata
     4921 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
     4981 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
     5041 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
     5101 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
     5161 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
     5221 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
     5281 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
     5341 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
     5401 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
     5461 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
     5521 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
     5581 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
     5641 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
     5701 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgtcaac
     5761 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
     5821 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
     5881 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5941 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     6001 tgagttagcg cgaattgatc tg
//
