LOCUS 40924_43923 6022 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pTrc-TrxTAL, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6022) AUTHORS Kim B, Binkley R, Kim HU, Lee SY. TITLE Metabolic engineering of Escherichia coli for the enhanced production of l-tyrosine JOURNAL Biotechnol. Bioeng. (2018) In press PUBMED 30019750 REFERENCE 2 (bases 1 to 6022) AUTHORS Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY. TITLE Direct Submission JOURNAL Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon 34141, South Korea REFERENCE 3 (bases 1 to 6022) TITLE Direct Submission REFERENCE 4 (bases 1 to 6022) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol. Bioeng. (2018) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon 34141, South Korea" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6022 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 193..222 /label=trc promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" protein_bind 230..246 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." 5'UTR 247..271 /gene="TAL" gene 247..271 /gene="TAL" /label=TAL CDS 272..598 /label=TrxA /note="E. coli thioredoxin" misc_feature 599..2128 /gene="TAL" /note="similar to tyrosine ammonia-lyase; derived from Saccharothrix espanaensis" gene 599..2128 /gene="TAL" /label=TAL terminator 2375..2461 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 2553..2580 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" promoter 2600..2691 /label=AmpR promoter CDS 2692..3549 /label=AmpR /note="beta-lactamase" rep_origin 3723..4311 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(4497..4637) /label=bom /note="basis of mobility region from pBR322" promoter 4823..4900 /label=lacIq promoter /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." CDS 4901..5980 /label=lacI /note="lac repressor" ORIGIN 1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 241 taacaaagac cgaggaaaag gagcatcgca aatgagcgat aaaattattc acctgactga 301 cgacagtttt gacacggatg tactcaaagc ggacggggcg atcctcgtcg atttctgggc 361 agagtggtgc ggtccgtgca aaatgatcgc cccgattctg gatgaaatcg ctgacgaata 421 tcagggcaaa ctgaccgttg caaaactgaa catcgatcaa aaccctggca ctgcgccgaa 481 atatggcatc cgtggtatcc cgactctgct gctgttcaaa aacggtgaag tggcggcaac 541 caaagtgggt gcactgtcta aaggtcagtt gaaagagttc ctcgacgcta acctggcgac 601 gcaggtcgtg gaacgtcagg ctgatcggct cagcagcagg gagtacctgg cccgggtcgt 661 gcgcagcgcc gggtgggacg ccggtctcac ctcgtgcacc gacgaggaga tcgtccggat 721 gggcgcgagc gcgcgcacca tcgaggagta cctgaagtcc gacaagccca tctacggcct 781 gacgcagggc ttcggtccgc tggtgctgtt cgacgccgac tcggagctgg agcagggcgg 841 ctcgctgatc tcgcacctgg gcaccggcca gggcgcgcca ctggccccgg aggtgtcgcg 901 gctgatcctc tggctgcgca tccagaacat gcgcaagggg tactcggcgg tctcgccggt 961 gttctggcag aagctcgccg acctgtggaa caaggggttc accccggcga tcccccggca 1021 cggcacggtc agcgcgagcg gcgacctgca accgctggcg cacgccgcgc tcgccttcac 1081 cggtgtcggc gaggcgtgga cccgggacgc cgacggccgg tggtccaccg tgccggccgt 1141 ggacgcgctc gccgcgctgg gggcggagcc gttcgactgg ccggtgcgcg aggcgctggc 1201 gttcgtcaac gggaccggcg cgagcctcgc ggtggctgtg ctcaaccacc ggtccgccct 1261 gcggctggtc cgcgcctgcg ccgtgctctc cgcgcggctg gcgaccctgc tgggggccaa 1321 tcccgagcac tacgacgtgg ggcacggtgt cgcgcgcggc caggtcggtc agctgaccgc 1381 ggcggagtgg atccggcagg ggctgccccg gggcatggtg cgcgacggca gccgcccgct 1441 ccaggagccg tacagcctgc ggtgcgcgcc gcaggtgctc ggcgcggtgc tcgaccagct 1501 cgacggcgcg ggcgacgtgc tggcgcggga ggtcgacggc tgccaggaca acccgatcac 1561 ctacgagggc gagctgctgc acggcggcaa cttccacgcc atgccggtgg gtttcgcctc 1621 cgaccagatc gggttggcca tgcacatggc cgcctacctg gccgagcgcc agctgggtct 1681 gctggtcagc ccggtgacca acggcgacct gccgcccatg ctcaccccgc gcgccgggcg 1741 cggtgccggg ctggccgggg tgcagatcag cgcgacctcg ttcgtctcgc ggatccggca 1801 gctggtgttc cccgcctcgc tgaccaccct gccgaccaac ggctggaacc aggaccacgt 1861 gccgatggcg ctcaacgggg cgaactcggt gttcgaggcg ttggagctcg gctggctgac 1921 ggtcgggtcg ctggcggtgg gcgtcgcgca gctcgcggcc atgaccggcc acgccgcgga 1981 gggcgtctgg gcggagctgg ccgggatctg cccgccgctg gacgccgacc gcccgctggg 2041 cgccgaggtg cgcgccgcgc gcgacctgct gtccgcgcac gcggaccaac tgctcgtcga 2101 cgaggcagac gggaaggatt tcggatgagt acccggggat cctctagagt cgacctgcag 2161 gcatgcaagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa 2221 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc 2281 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg 2341 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa 2401 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa 2461 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg 2521 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt 2581 tgcgtttcta caaactcttt ttgtttattt ttctaaatac attcaaatat gtatccgctc 2641 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 2701 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 2761 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 2821 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 2881 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac 2941 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 3001 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 3061 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 3121 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 3181 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctacagca 3241 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 3301 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 3361 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 3421 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 3481 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 3541 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 3601 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 3661 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 3721 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 3781 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 3841 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac 3901 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 3961 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 4021 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 4081 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 4141 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 4201 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 4261 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 4321 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 4381 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 4441 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg 4501 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc 4561 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg 4621 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt 4681 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc 4741 agaggttttc accgtcatca ccgaaacgcg cgaggcagca gatcaattcg cgcgcgaagg 4801 cgaagcggca tgcatttacg ttgacaccat cgaatggtgc aaaacctttc gcggtatggc 4861 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata 4921 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc 4981 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta 5041 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc 5101 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc 5161 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg 5221 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc 5281 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt 5341 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac 5401 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg 5461 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg 5521 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca 5581 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga 5641 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga 5701 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgtcaac 5761 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact 5821 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa 5881 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 5941 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 6001 tgagttagcg cgaattgatc tg //