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pTransgene vector (V002558)

Basic Vector Information

Vector Name:
pTransgene
Antibiotic Resistance:
Ampicillin
Length:
5398 bp
Type:
Cloning vector
Replication origin:
ori
Source/Author:
Yang X, Pachikara B, Murray TJ, Liu D, Sonenshein GE, Sherr DH.

pTransgene vector Vector Map

pTransgene5398 bp6001200180024003000360042004800T7 promoterchicken beta-globin core insulator; shields transgene from chromosomal positional effectsPacI and AvrII restriction sites for changing promoterSacII and SpeI restriction sites for changing promoterchimeric intronmultiple cloning site; contains MluI, XbaI, SalI, AccI, EagI and NotI restriction sitesSV40 poly(A) signalchicken beta-globin core insulator; shields transgene from chromosomal positional effectsSphI and SbfI restriction sites to isolate transgene constructf1 oriAmpR promoterAmpRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTransgene vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_43858        5398 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTransgene, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Yang X, Pachikara B, Murray TJ, Liu D, Sonenshein GE, Sherr DH.
  TITLE     pTransgene: A cloning vector with insulators for optimal cDNA 
            transgene expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Yang X, Sonenshein GE, Sherr DH.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2002) Environmental Health, Boston University 
            School of Public Health and Medicine, 715 Albany Street, R-405, 
            Boston, MA 02118, USA
REFERENCE   3  (bases 1 to 5398)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5398)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAY-2002) Environmental Health, Boston University School of 
            Public Health and Medicine, 715 Albany Street, R-405, Boston, MA 
            02118, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..40
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    62..635
                     /note="chicken beta-globin core insulator; shields
                     transgene from chromosomal positional effects"
     misc_feature    647..707
                     /note="PacI and AvrII restriction sites for changing
                     promoter"
     regulatory      668..1948
                     /label=mouse mammary tumor virus 5' LTR
                     /note="mouse mammary tumor virus 5' LTR"
                     /regulatory_class="promoter"
     misc_feature    1949..1960
                     /note="SacII and SpeI restriction sites for changing
                     promoter"
     intron          1986..2118
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    2146..2181
                     /note="multiple cloning site; contains MluI, XbaI, SalI,
                     AccI, EagI and NotI restriction sites"
     polyA_signal    complement(2197..2318)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2424..2678
                     /note="chicken beta-globin core insulator; shields
                     transgene from chromosomal positional effects"
     misc_feature    2702..2714
                     /note="SphI and SbfI restriction sites to isolate transgene
                     construct"
     rep_origin      2799..3254
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3586..3690
                     /label=AmpR promoter
     CDS             3691..4548
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4722..5310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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