LOCUS       40924_43858        5398 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTransgene, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Yang X, Pachikara B, Murray TJ, Liu D, Sonenshein GE, Sherr DH.
  TITLE     pTransgene: A cloning vector with insulators for optimal cDNA 
            transgene expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Yang X, Sonenshein GE, Sherr DH.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2002) Environmental Health, Boston University 
            School of Public Health and Medicine, 715 Albany Street, R-405, 
            Boston, MA 02118, USA
REFERENCE   3  (bases 1 to 5398)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5398)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAY-2002) Environmental Health, Boston University School of 
            Public Health and Medicine, 715 Albany Street, R-405, Boston, MA 
            02118, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..40
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    62..635
                     /note="chicken beta-globin core insulator; shields
                     transgene from chromosomal positional effects"
     misc_feature    647..707
                     /note="PacI and AvrII restriction sites for changing
                     promoter"
     regulatory      668..1948
                     /label=mouse mammary tumor virus 5' LTR
                     /note="mouse mammary tumor virus 5' LTR"
                     /regulatory_class="promoter"
     misc_feature    1949..1960
                     /note="SacII and SpeI restriction sites for changing
                     promoter"
     intron          1986..2118
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    2146..2181
                     /note="multiple cloning site; contains MluI, XbaI, SalI,
                     AccI, EagI and NotI restriction sites"
     polyA_signal    complement(2197..2318)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2424..2678
                     /note="chicken beta-globin core insulator; shields
                     transgene from chromosomal positional effects"
     misc_feature    2702..2714
                     /note="SphI and SbfI restriction sites to isolate transgene
                     construct"
     rep_origin      2799..3254
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3586..3690
                     /label=AmpR promoter
     CDS             3691..4548
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4722..5310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatcttaag gctagagtac ttaatacgac tcactatagg ctagcctcga gaattcgagt
       61 tggcgcgcct gtcattctaa atctctcttt cagcctaaag ctttttcccc gtatcccccc
      121 aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac
      181 cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg
      241 gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta
      301 catccctggg ggctttggga gggggctgtc cccgtgagct cccaggcgcg cctgtcattc
      361 taaatctctc tttcagccta aagctttttc cccgtatccc cccaggtgtc tgcaggctca
      421 aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc
      481 tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc
      541 gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg
      601 ggggggggct gtccccgtga gctcccaggc gcgccaactc ggtaccttaa ttaatgccgc
      661 gcctgcagca gaaatggttg aactcccgag agtgtcctac acctagggga gaagcagcca
      721 aggggttgtt tcccaccaag gacgacccgt ctgcgcacaa acggatgagc ccatcagaca
      781 aagacatatt cattctctgc tgcaaacttg gcatagctct gctttgcctg gggctattgg
      841 gggaagttgc ggttcgtgct cgcagggctc tcacccttga ctcttctaat agctcttctg
      901 tgcaagatta caatctaaac aattcggaga actcgacctt cctcctgagg caaggaccac
      961 agccaacttc ctcttacaag ccgcatcgat tttgtccttc agaaatagaa ataagaatgc
     1021 ttgctaaaaa ttatattttt accaataaga ccaatccaat aggtagatta ttagttacta
     1081 tgttaagaaa tgaatcatta tcttttagta ctatttttac tcaaattcag aagttagaaa
     1141 tgggaataga aaatagaaag agacgctcaa cctcaattga agaacaggtg caaggactat
     1201 tgaccacagg cctagaagta aaaaagggaa aaaagagtgt ttttgtcaaa ataggagaca
     1261 ggtggtggca cccagggact tataggggac cttacatcta cagaccaaca gatgccccct
     1321 ttaccatata caggaagata tgacttaaat tgggataggt gggttacagt caatggctat
     1381 aaagtgttat atagatccct ccccttttcg tgaaagactc gccagagcta gacctccttg
     1441 gtgtatgttg tctcaagaag aaaaagacga catgaaacaa caggtacatg attatattta
     1501 tctaggaaca ggaatgcact tttggggaaa gattttccat accaaggagg ggacagtggc
     1561 tggactaata gaacattatt ctgcaaaaac ttatggcatg agttattatg aatagccttt
     1621 attggcccaa ccttgcggtt cccagggctt aagtaagttt ttggttacaa actgttctta
     1681 aaacgaggat gtgagacaag tggtttcctg acttggtttg gtatcaaagg ttctgatctg
     1741 agctctgagt gttctatttt cctatgttct tttggaattt atccaaatct tatgtaaatg
     1801 cttatgtaaa ccaagatata aaagagtgct gattttttga gtaaacttgc aacagtccta
     1861 acattcacct cttgtgtgtt tgtgtctgtt cgccatcccg tctccgctcg tcacttatcc
     1921 ttcactttcc agagggtccc cccgcagacc gcggactagt aagttggtcg tgaggcactg
     1981 ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcttgt
     2041 cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact gacatccact
     2101 ttgcctttct ctccacaggt gtccactccc agttcaatta cagctacgcg tggtacctct
     2161 agagtcgacc cgggcggccg cttcgagcag acatgataag atacattgat gagtttggac
     2221 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     2281 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     2341 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca
     2401 aatgtggtaa aatcgataag gatcagcttt ttccccgtat ccccccaggt gtctgcaggc
     2461 tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg
     2521 ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc
     2581 cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct
     2641 ttgggggggg gctgtccccg tgagctccca ggcgcgccaa ctcggtaccc ggggatctcg
     2701 acctgcaggc atgcaagctg gttccgggct ggcgtgatag cgaagaggcc cgcaccgatc
     2761 gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     2821 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     2881 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     2941 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3001 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3061 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3121 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     3181 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     3241 aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     3301 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     3361 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3421 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     3481 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     3541 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3601 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3661 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3721 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3781 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3841 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     3901 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     3961 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4021 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4081 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4141 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4201 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4261 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4321 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4381 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4441 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4501 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4561 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4621 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4681 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4741 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4801 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4861 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4921 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4981 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5041 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5101 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5161 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5221 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5281 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5341 ctggcctttt gctggccttt tgctcacatg gctcgacaga tcttaaggct agagtact
//
