pTRA vector (V002559)

LOCUS       40924_43848        4286 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTRA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Wagner SG, Ziegler M, Kremling A, Pflueger-Grau K.
  TITLE     pTRA - a reporter system for the quantitative characterisation of 
            synthetic building blocks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Wagner SG, Ziegler M, Kremling A, Pflueger-Grau K.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2017) Systems Biotechnology, Technical University 
            Munich, Boltzmannstr. 15, Garching 85748, Germany
REFERENCE   3  (bases 1 to 4286)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4286)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-OCT-2017) Systems Biotechnology, Technical University Munich, 
            Boltzmannstr. 15, Garching 85748, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      95..108
                     /regulatory_class="ribosome_binding_site"
     misc_feature    126..359
                     /label=F30-2xdBoccoli mRNA-tag
                     /note="F30-2xdBoccoli mRNA-tag"
     regulatory      371..384
                     /regulatory_class="ribosome_binding_site"
     CDS             399..419
                     /label=7xHis
                     /note="6xHis affinity tag"
     CDS             complement(463..480)
                     /label=tetracysteine tag
                     /note="tetracysteine peptide that binds biarsenical
                     labeling reagents"
     CDS             699..716
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    735..968
                     /function="F30-2xdBroccoli mRNA-tag"
     terminator      993..1087
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1118..1146
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1335..1865
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     oriT            2079..2187
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2281..2869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2926..3756)
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      3770..4121
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(4194..4280)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"