LOCUS       40924_43848        4286 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTRA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Wagner SG, Ziegler M, Kremling A, Pflueger-Grau K.
  TITLE     pTRA - a reporter system for the quantitative characterisation of 
            synthetic building blocks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Wagner SG, Ziegler M, Kremling A, Pflueger-Grau K.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2017) Systems Biotechnology, Technical University 
            Munich, Boltzmannstr. 15, Garching 85748, Germany
REFERENCE   3  (bases 1 to 4286)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4286)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-OCT-2017) Systems Biotechnology, Technical University Munich, 
            Boltzmannstr. 15, Garching 85748, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      95..108
                     /regulatory_class="ribosome_binding_site"
     misc_feature    126..359
                     /label=F30-2xdBoccoli mRNA-tag
                     /note="F30-2xdBoccoli mRNA-tag"
     regulatory      371..384
                     /regulatory_class="ribosome_binding_site"
     CDS             399..419
                     /label=7xHis
                     /note="6xHis affinity tag"
     CDS             complement(463..480)
                     /label=tetracysteine tag
                     /note="tetracysteine peptide that binds biarsenical
                     labeling reagents"
     CDS             699..716
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    735..968
                     /function="F30-2xdBroccoli mRNA-tag"
     terminator      993..1087
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1118..1146
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1335..1865
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     oriT            2079..2187
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2281..2869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2926..3756)
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      3770..4121
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(4194..4280)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagg gcccaagaag gagatatacc atggaaagcg
      121 gccgcttgcc atgtgtatgt gggagacggt cgggtccatc tgagacggtc gggtccagat
      181 attcgtatct gtcgagtaga gtgtgggctc agatgtcgag tagagtgtgg gctcccacat
      241 actctgatga tccagacggt cgggtccatc tgagacggtc gggtccagat attcgtatct
      301 gtcgagtaga gtgtgggctc agatgtcgag tagagtgtgg gctggatcat tcatggcaat
      361 gagcggccgc aagaaggaga tataccatga gcagcagcca tcatcatcat catcaccata
      421 tgggcgatcc gaaactgctg ctggaagtgc cgcatgcgat tagcagcagc ccgggcaaca
      481 gcctggcggt ggtgctacag cgccgcgatt gggaaaaccc gggcgtgacc cagctgaacc
      541 gcctggcggc gcatccgccg tttgcgagct ggcgcaacag cgaagaagcg cgcaccgatc
      601 gcccgagcca gcagctgcgc agcctgattc gcctgctgac caaaccggaa cgcaaactga
      661 gctggctgct gccgccgctg agcaacaact gactcgagca ccaccaccac caccactgat
      721 catgaggcgg ccgcttgcca tgtgtatgtg ggagacggtc gggtccatct gagacggtcg
      781 ggtccagata ttcgtatctg tcgagtagag tgtgggctca gatgtcgagt agagtgtggg
      841 ctcccacata ctctgatgat ccagacggtc gggtccatct gagacggtcg ggtccagata
      901 ttcgtatctg tcgagtagag tgtgggctca gatgtcgagt agagtgtggg ctggatcatt
      961 catggcaatg agcggccgct tgactagtct tggactcctg ttgatagatc cagtaatgac
     1021 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg
     1081 tgagaatcca ggggtcccca ataattacga tttaaatttg acataagcct gttcggttcg
     1141 taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     1201 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca
     1261 gcctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     1321 atggagcagc aacgatgtta cgcagcagca acgatgttac gcagcagggc agtcgcccta
     1381 aaacaaagtt aggtggctca agtatgggca tcattcgcac atgtaggctc ggccctgacc
     1441 aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcgga gacgtagcca
     1501 cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt agtaagacat
     1561 tcatcgcgct tgctgccttc gaccaagaag cggttgttgg cgctctcgcg gcttacgttc
     1621 tgcccaagtt tgagcagccg cgtagtgaga tctatatcta tgatctcgca gtctccggag
     1681 agcaccggag gcagggcatt gccaccgcgc tcatcaatct cctcaagcat gaggccaacg
     1741 cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg tgacgatccc gcagtggctc
     1801 tctatacaaa gttgggcata cgggaagaag tgatgcactt tgatatcgac ccaagtaccg
     1861 ccacctaaca attcgttcaa gccgagatcg gcttcccggc cgcggagttg ttcggtaaat
     1921 tggacaacgg tccgcgcgtt gtccttttcc gctgcataac cctgcttcgg ggtcattata
     1981 gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt
     2041 cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc
     2101 ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac
     2161 gggaatcctg ctctgcgagg ctggccgtag gccggccgat aatctcatga ccaaaatccc
     2221 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2281 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2341 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2401 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2461 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2521 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2581 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2641 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2701 gagaaaggcg gacaggcatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2761 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2821 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2881 cgcggccgtg aaaggcaggc cggtccgtgg tggccacggc ctctaggcca gatccagcgg
     2941 catctgggtt agtcgagcgc gggccgcttc ccatgtctca ccagggcgag cctgtttcgc
     3001 gatctcagca tctgaaatct tcccggcctt gcgcttcgct ggggccttac ccaccgcctt
     3061 ggcgggcttc ttcggtccaa aactgaacaa cagatgtgtg accttgcgcc cggtctttcg
     3121 ctgcgcccac tccacctgta gcgggctgtg ctcgttgatc tgcgtcacgg ctggatcaag
     3181 cactcgcaac ttgaagtcct tgatcgaggg ataccggcct tccagttgaa accactttcg
     3241 cagctggtca atttctattt cgcgctggcc gatgctgtcc cattgcatga gcagctcgta
     3301 aagcctgatc gcgtgggtgc tgtccatctt ggccacgtca gccaaggcgt atttggtgaa
     3361 ctgtttggtg agttccgtca ggtacggcag catgtctttg gtgaacctga gttctacacg
     3421 gccctcaccc tcccggtaga tgattgtttg cacccagccg gtaatcatca cactcggtct
     3481 tttccccttg ccattgggct cttgggttaa ccggacttcc cgccgtttca ggcgcagggc
     3541 cgcttctttg agctggttgt aggaagattc gatagggaca cccgccatcg tcgctatgtc
     3601 ctccgccgtc actgaataca tcacttcatc ggtgacaggc tcgctcctct tcacctggct
     3661 aatacaggcc agaacgatcc gctgttcctg aacactgagg cgatacgcgg cctcgaccag
     3721 ggcattgctt ttgtaaacca ttgggggtga ggccacgttc gacattcctt gtgtataagg
     3781 ggacactgta tctgcgtccc acaatacaac aaatccgtcc ctttacaaca acaaatccgt
     3841 cccttcttaa caacaaatcc gtcccttaat ggcaacaaat ccgtcccttt ttaaactcta
     3901 caggccacgg attacgtggc ctgtagacgt cctaaaaggt ttaaaaggga aaaggaagaa
     3961 aagggtggaa acgcaaaaaa cgcaccacta cgtggccccg ttggggccgc atttgtgccc
     4021 ctgaaggggc gggggaggcg tctgggcaat ccccgtttta ccagtcccct atcgccgcct
     4081 gagagggcgc aggaagcgag taatcagggt atcgaggcgg attcaccctt ggcgtccaac
     4141 cagcggcacc agcggcgcct gagaggggcg cgcccagctg tctagggcgg cggatttgtc
     4201 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     4261 tgagcctttc gttttatttg atgcct
//
