Basic Vector Information
- Vector Name:
- pBAD-mNectarine
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4782 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- E. coli
- Promoter:
- BAD
- 5' Primer:
- rrnBT1-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pBAD-mNectarine vector Map
pBAD-mNectarine vector Sequence
LOCUS . 4782 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
CDS complement(120..977)
/label="AmpR"
/note="β-lactamase"
promoter complement(978..1069)
/label="AmpR promoter"
terminator complement(1088..1115)
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator complement(1207..1293)
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(1512..2219)
/label="mNectarine"
/note="pH-sensitive monomeric red fluorescent protein
derived from DsRed and mTangerine (Johnson et al., 2009)"
CDS complement(2235..2258)
/label="Xpress™ tag"
/note="Xpress™ epitope tag, including an enterokinase
recognition and cleavage site"
CDS complement(2298..2315)
/label="6xHis"
/note="6xHis affinity tag"
promoter complement(2361..2645)
/label="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
CDS 2672..3547
/label="araC"
/note="L-arabinose regulatory protein"
misc_feature 3814..3954
/label="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(4140..4728)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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