LOCUS       .                       4782 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(120..977)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(978..1069)
                     /label="AmpR promoter"
     terminator      complement(1088..1115)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(1207..1293)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1512..2219)
                     /label="mNectarine"
                     /note="pH-sensitive monomeric red fluorescent protein
                     derived from DsRed and mTangerine (Johnson et al., 2009)"
     CDS             complement(2235..2258)
                     /label="Xpress™ tag"
                     /note="Xpress™ epitope tag, including an enterokinase
                     recognition and cleavage site"
     CDS             complement(2298..2315)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     promoter        complement(2361..2645)
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             2672..3547
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     misc_feature    3814..3954
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4140..4728)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
       61 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
      121 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
      181 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
      241 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
      301 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
      361 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
      421 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      481 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      541 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
      601 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      661 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      721 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
      781 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      841 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      901 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      961 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     1021 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaaa gagtttgtag
     1081 aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc tggcagttta
     1141 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca aatccgctcc
     1201 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     1261 ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcat
     1321 ggggagaccc cacactacca tcggcgctac ggcgtttcac ttctgagttc ggcatggggt
     1381 caggtgggac caccgcgcta ctgccgccag gcaaattctg ttttatcaga ccgcttctgc
     1441 gttctgattt aatctgtatc aggctgaaaa tcttctctca tccgccaaaa cagccaagct
     1501 tcgaattctt acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcagcgcgt
     1561 tcgtacagtt ccacgatggt gtagtcctcg ttgtgggaaa ggatgtccaa cttgatgtcg
     1621 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca
     1681 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgca tgatctcgcc cttcagggcg
     1741 ccgtcctcgg ggtgcatccg ctcagtggag gcctcccagc ccacggtacg acactgcatt
     1801 acggggccgt cggaggggaa gttagtgccg cgcagcttca ccttgtagat gaactcgccg
     1861 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
     1921 cgctcccaat tgaggccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
     1981 tgcttcacat aggccttgga gccgtagcag aactgagggg acaggatgtc ccaggcgaag
     2041 ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
     2101 ccctcgccct caccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc
     2161 ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccatg
     2221 ctcgagctcg gatccttatc gtcatcgtcg tacagatccc gacccatttg ctgtccacca
     2281 gtcatgctag ccataccatg atgatgatga tgatgagaac cccccatggt taattcctcc
     2341 tgttagccca aaaaacgggt atggagaaac agtagagagt tgcgataaaa agcgtcaggt
     2401 aggatccgct aatcttatgg ataaaaatgc tatggcatag caaagtgtga cgccgtgcaa
     2461 ataatcaatg tggacttttc tgccgtgatt atagacactt ttgttacgcg tttttgtcat
     2521 ggctttggtc ccgctttgtt acagaatgct tttaataagc ggggttaccg gtttggttag
     2581 cgagaagagc cagtaaaaga cgcagtgacg gcaatgtctg atgcaatatg gacaattggt
     2641 ttcttctctg aatggcggga gtatgaaaag tatggctgaa gcgcaaaatg atcccctgct
     2701 gccgggatac tcgtttaatg cccatctggt ggcgggttta acgccgattg aggccaacgg
     2761 ttatctcgat ttttttatcg accgaccgct gggaatgaaa ggttatattc tcaatctcac
     2821 cattcgcggt cagggggtgg tgaaaaatca gggacgagaa tttgtttgcc gaccgggtga
     2881 tattttgctg ttcccgccag gagagattca tcactacggt cgtcatccgg aggctcgcga
     2941 atggtatcac cagtgggttt actttcgtcc gcgcgcctac tggcatgaat ggcttaactg
     3001 gccgtcaata tttgccaata cggggttctt tcgcccggat gaagcgcacc agccgcattt
     3061 cagcgacctg tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct attcggagct
     3121 gctggcgata aatctgcttg agcaattgtt actgcggcgc atggaagcga ttaacgagtc
     3181 gctccatcca ccgatggata atcgggtacg cgaggcttgt cagtacatca gcgatcacct
     3241 ggcagacagc aattttgata tcgccagcgt cgcacagcat gtttgcttgt cgccgtcgcg
     3301 tctgtcacat cttttccgcc agcagttagg gattagcgtc ttaagctggc gcgaggacca
     3361 acgtatcagc caggcgaagc tgcttttgag caccacccgg atgcctatcg ccaccgtcgg
     3421 tcgcaatgtt ggttttgacg atcaactcta tttctcgcgg gtatttaaaa aatgcaccgg
     3481 ggccagcccg agcgagttcc gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa
     3541 gttgtcataa ttggtaacga atcagacaat tgacggcttg acggagtagc atagggtttg
     3601 cagaatccct gcttcgtcca tttgacaggc acattatgca tgccgcttcg ccttcgcgcg
     3661 cgaattgatc tgctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     3721 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     3781 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     3841 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     3901 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     3961 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4021 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4081 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4141 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4201 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4261 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4321 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4381 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4441 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4501 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4561 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4621 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4681 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4741 atcttttcta cggggtctga cgctcagtgg aacgaaaact ca
//
