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pscAAV8-GFP vector (V018039)

Basic Vector Information

Vector Name:
pscAAV8-GFP
Antibiotic Resistance:
Ampicillin
Length:
4573 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells, Adeno-associated virus
Promoter:
CMV
5' Primer:
CMV-F
Growth Strain(s):
Stbl3
Growth Temperature:
37℃

pscAAV8-GFP vector Map

Copy Sequence View Map
pscAAV8-GFP4573 bp600120018002400300036004200CMV enhancerCMV promoterMCSEGFPSV40 poly(A) signalAAV2 ITRf1 oriAmpR promoterAmpRori

pscAAV8-GFP vector Sequence

LOCUS       .                       4573 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        209..512
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        513..716
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    739..819
                     /label="MCS"
                     /note="multiple cloning site"
     CDS             827..1543
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     polyA_signal    1669..1803
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     repeat_region   1836..1976
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     rep_origin      2051..2506
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2788..2892
                     /label="AmpR promoter"
     CDS             2893..3750
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      3924..4512
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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