LOCUS       .                       4573 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        209..512
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        513..716
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    739..819
                     /label="MCS"
                     /note="multiple cloning site"
     CDS             827..1543
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     polyA_signal    1669..1803
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     repeat_region   1836..1976
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     rep_origin      2051..2506
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2788..2892
                     /label="AmpR promoter"
     CDS             2893..3750
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      3924..4512
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtgggcgg ccgcggccgc
      121 acgcgtcgac ggatccgaat tcgatatcgc tagcataata gtaatcaatt acggggtcat
      181 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      241 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      301 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      361 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      421 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      481 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      541 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      601 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      661 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
      721 tagtgaaccg tcagatccgc tagcgctacc ggactcagat ctcgagctca agcttcgaat
      781 tctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg
      841 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      901 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      961 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1021 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     1081 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1141 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1201 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1261 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     1321 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     1381 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     1441 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1501 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc
     1561 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     1621 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     1681 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     1741 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta agatatctag
     1801 atctcgaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
     1861 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     1921 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     1981 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     2041 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     2101 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     2161 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     2221 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     2281 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     2341 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     2401 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     2461 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     2521 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     2581 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     2641 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     2701 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     2761 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2821 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2881 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     2941 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3001 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     3061 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     3121 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     3181 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     3241 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     3301 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3361 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     3421 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     3481 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     3541 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     3601 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     3661 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     3721 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     3781 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3841 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3901 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3961 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4021 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     4081 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4141 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4201 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4261 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4321 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4381 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4441 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4501 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4561 tttgctcaca tgt
//
