Basic Vector Information
- Vector Name:
- pCI-3×FLAG-MCS-Neo-2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5549 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- CMV
- 5' Primer:
- CMV-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pCI-3×FLAG-MCS-Neo-2 vector Map
pCI-3×FLAG-MCS-Neo-2 vector Sequence
LOCUS . 5549 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
enhancer 139..517
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 518..729
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 890..1022
/label="chimeric intron"
/note="chimera between introns from human β-globin and
immunoglobulin heavy chain genes"
promoter 1067..1085
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1102..1167
/label="3xFLAG"
/note="three tandem FLAG® epitope tags, followed by an
enterokinase cleavage site"
polyA_signal complement(1253..1374)
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 1560..2015
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2132..2489
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 2540..3331
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
polyA_signal 3398..3446
/label="poly(A) signal"
/note="synthetic polyadenylation signal"
promoter 3752..3856
/label="AmpR promoter"
CDS 3857..4714
/label="AmpR"
/note="β-lactamase"
rep_origin 4888..5476
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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