LOCUS       .                       5549 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        139..517
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          890..1022
                     /label="chimeric intron"
                     /note="chimera between introns from human β-globin and
                     immunoglobulin heavy chain genes"
     promoter        1067..1085
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1102..1167
                     /label="3xFLAG"
                     /note="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
     polyA_signal    complement(1253..1374)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      1560..2015
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2132..2489
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2540..3331
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3398..3446
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     promoter        3752..3856
                     /label="AmpR promoter"
     CDS             3857..4714
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      4888..5476
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc gcgccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac
     1141 atcgattaca aggatgacga tgacaagctc gagaattcac gcgtggtacc tctagagtcg
     1201 acccgggcgg ccgcttccct ttagtgaggg ttaatgcttc gagcagacat gataagatac
     1261 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     1321 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     1381 aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc
     1441 aagtaaaacc tctacaaatg tggtaaaatc cgataaggat cgatccgggc tggcgtaata
     1501 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
     1561 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     1621 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     1681 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     1741 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     1801 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     1861 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     1921 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     1981 cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg
     2041 catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc agcaccatgg cctgaaataa
     2101 cctctgaaag aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg
     2161 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2221 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     2281 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     2341 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     2401 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     2461 gcttttttgg aggcctaggc ttttgcaaaa agcttgattc ttctgacaca acagtctcga
     2521 acttaaggct agagccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     2581 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     2641 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     2701 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     2761 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     2821 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     2881 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     2941 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     3001 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     3061 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     3121 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     3181 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     3241 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     3301 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     3361 gaccaagcga cgcccaacct gccatcacga tggccgcaat aaaatatctt tattttcatt
     3421 acatctgtgt gttggttttt tgtgtgaatc gatagcgata aggatccgcg tatggtgcac
     3481 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     3541 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     3601 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     3661 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     3721 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3781 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3841 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3901 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3961 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4021 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     4081 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     4141 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4201 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4261 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4321 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4381 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4441 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4501 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4561 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4621 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4681 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4741 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4801 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4861 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4921 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4981 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     5041 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5101 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5161 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5221 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5281 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5341 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5401 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5461 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5521 gccttttgct cacatggctc gacagatct
//
