Basic Vector Information
- Vector Name:
- pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q-T375G)-delta-984-1090-CMV-EGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 11051 bp
- Type:
- Gene-editing vector
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- CMV
- 3' Primer:
- BGH-R
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q-T375G)-delta-984-1090-CMV-EGFP vector Map
pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q-T375G)-delta-984-1090-CMV-EGFP vector Sequence
LOCUS . 11051 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
CDS 1241..1267
/label="NES"
/note="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
enhancer 2455..2834
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 2835..3038
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 3083..3101
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 3222..3938
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
polyA_signal 4005..4229
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 4275..4703
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4717..5046
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 5113..5904
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
polyA_signal 6081..6214
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter complement(6299..6329)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(6344..6365)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6653..7241)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7415..8272)
/label="AmpR"
/note="β-lactamase"
promoter complement(8273..8377)
/label="AmpR promoter"
enhancer 8643..9020
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 9023..9226
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
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