LOCUS       .                      11051 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1241..1267
                     /label="NES"
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     enhancer        2455..2834
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2835..3038
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        3083..3101
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3222..3938
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     polyA_signal    4005..4229
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4275..4703
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4717..5046
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5113..5904
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    6081..6214
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(6299..6329)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6344..6365)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6653..7241)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7415..8272)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(8273..8377)
                     /label="AmpR promoter"
     enhancer        8643..9020
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9023..9226
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
ORIGIN
        1 attcaaggac gaccggaagt ccattcggag cgccgacaac aagatgggaa agagaggctt
       61 caagcagatc tccacaggca agctggccga cttcctggcc aaggacatcg tgctgtttca
      121 gcccagcgtg aacgatggcg agaacaagat caccggcctg aactaccgga tcatgcagag
      181 cgccattgcc gtgtacgata gcggcgacga ttacgaggcc aagcagcagt tcaagctgat
      241 gttcgagaag gcccggctga tcggcaaggg cacaacagag cctcatccat ttctgtacaa
      301 ggtgttcgcc cgcagcatcc ccgccaatgc cgtcgagttc tacgagcgct acctgatcga
      361 gcggaagttc tacctgaccg gcctgtccaa cgagatcaag aaaggcaaca gagtggatgt
      421 gcccttcatc cggcgggacc agaacaagtg gaaaacaccc gccatgaaga ccctgggcag
      481 aatctacagc gaggatctgc ccgtggaact gcccagacag atgttcgaca atgagatcaa
      541 gtcccacctg aagtccctgc cacagatgga aggcatcgac ttcaacaatg ccaacgtgac
      601 ctatctgatc gccgagtaca tgaagagagt gctggacgac gacttccaga ccttctacca
      661 gtggaaccgc aactaccggt acatggacat gcttaagggc gagtacgaca gaaagggctc
      721 cctgcagcac tgcttcacca gcgtggaaga gagagaaggc ctctggaaag agcgggcctc
      781 cagaacagag cggtacagaa agcaggccag caacaagatc cgcagcaacc ggcagatgag
      841 aaacgccagc agcgaagaga tcgagacaat cctggataag cggctgagca acagccggaa
      901 cgagtaccag aaaagcgaga aagtgatccg gcgctacaga gtgcaggatg ccctgctgtt
      961 tctgctggcc aaaaagaccc tgaccgaact ggccgatttc gacggcgaga ggttcaaact
     1021 gaaagaaatc atgcccgacg ccgagaaggg aatcctgagc gagatcatgc ccatgagctt
     1081 caccttcgag aaaggcggca agaagtacac catcaccagc gagggcatga agctgaagaa
     1141 ctacggcgac ttctttgtgc tggctagcga caagaggatc ggcaacctgc tggaactcgt
     1201 gggcagcgac atcgtgtcca aagaggatgg atcccttcaa ctgcctccac ttgaaagact
     1261 gacactggga tcccagctgc atttaccgca ggttttagct gacgctgtct cacgcctggt
     1321 cctgggtaag tttggtgacc tgaccgacaa cttctcctcc cctcacgctc gcagaaaagt
     1381 gctggctgga gtcgtcatga caacaggcac agatgttaaa gatgccaagg tgataagtgt
     1441 ttctacagga ggcaaatgta ttaatggtga atacatgagt gatcgtggcc ttgcattaaa
     1501 tgactgccat gcagaaataa tatctcggag atccttgctc agatttcttt atacacaact
     1561 tgagctttac ttaaataaca aagatgatca aaaaagatcc atctttcaga aatcagagcg
     1621 aggggggttt aggctgaagg agaatgtcca gtttcatctg tacatcagca cctctccctg
     1681 tggagatgcc agaatcttct caccacatga gccaatcctg gaagaaccag cagatagaca
     1741 cccaaatcgt aaagcaagag gacagctacg gaccaaaata gagtctggtc aggggacgat
     1801 tccagtgcgc tccaatgcga gcatccaaac gtgggacggg gtgctgcaag gggagcggct
     1861 gctcaccatg tcctgcagtg acaagattgc acgctggaac gtggtgggca tccagggatc
     1921 actgctcagc attttcgtgg agcccattta cttctcgagc atcatcctgg gcagccttta
     1981 ccacggggac cacctttcca gggccatgta ccagcggatc tccaacatag aggacctgcc
     2041 acctctctac accctcaaca agcctttgct cagtggcatc agcaatgcag aagcacggca
     2101 gccagggaag gcccccaact tcagtgtcaa ctggacggta ggcgactccg ctattgaggt
     2161 catcaacgcc acgactggga aggatgagct gggccgcgcg tcccgcctgt gtaagcacgc
     2221 gttgtactgt cgctggatgc gtgtgcacgg caaggttccc tcccacttac tacgctccaa
     2281 gattaccaag cccaacgtgt accatgagtc caagctggcg gcaaaggagt accaggccgc
     2341 caaggcgcgt ctgttcacag ccttcatcaa ggcggggctg ggggcctggg tggagaagcc
     2401 caccgagcag gaccagttct cactcacgta agcggccgcc cagatatacg cgttgacatt
     2461 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     2521 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2581 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2641 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2701 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2761 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2821 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2881 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2941 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3001 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
     3061 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
     3121 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
     3181 agtcgacggt accgcgggcc cgggatccac cggtcgccac catggtgagc aagggcgagg
     3241 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     3301 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     3361 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     3421 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3481 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     3541 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     3601 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     3661 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     3721 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     3781 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     3841 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     3901 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc tcgagtctag
     3961 agggcccgtt taagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     4021 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     4081 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     4141 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     4201 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     4261 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4321 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4381 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4441 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4501 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4561 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4621 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4681 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     4741 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4801 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4861 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4921 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4981 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5041 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     5101 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     5161 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     5221 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     5281 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     5341 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     5401 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     5461 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     5521 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     5581 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     5641 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     5701 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     5761 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     5821 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     5881 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     5941 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     6001 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     6061 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     6121 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6181 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     6241 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6301 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6361 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6421 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6481 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6541 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6601 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6661 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6721 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6781 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6841 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6901 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6961 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7021 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7081 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7141 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7201 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7261 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7321 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7381 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7441 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7501 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7561 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7621 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7681 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     7741 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7801 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7861 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7921 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     7981 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8041 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8101 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8161 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8221 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8281 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8341 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8401 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
     8461 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     8521 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     8581 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
     8641 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     8701 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     8761 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     8821 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     8881 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     8941 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     9001 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     9061 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     9121 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     9181 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag
     9241 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
     9301 tggctagcgt ttaaacttaa gcttgccacc atgaacatcc ccgctctggt ggaaaaccag
     9361 aagaagtact ttggcaccta cagcgtgatg gccatgctga acgctcagac cgtgctggac
     9421 cacatccaga aggtggccga tattgagggc gagcagaacg agaacaacga gaatctgtgg
     9481 tttcaccccg tgatgagcca cctgtacaac gccaagaacg gctacgacaa gcagcccgag
     9541 aaaaccatgt tcatcatcga gcggctgcag agctacttcc cattcctgaa gatcatggcc
     9601 gagaaccaga gagagtacag caacggcaag tacaagcaga accgcgtgga agtgaacagc
     9661 aacgacatct tcgaggtgct gaagcgcgcc ttcggcgtgc tgaagatgta cagggacctg
     9721 accaacgcat acaagaccta cgaggaaaag ctgaacgacg gctgcgagtt cctgaccagc
     9781 acagagcaac ctctgagcgg catgatcaac aactactaca cagtggccct gcggaacatg
     9841 aacgagagat acggctacaa gacagaggac ctggccttca tccaggacaa gcggttcaag
     9901 ttcgtgaagg acgcctacgg caagaaaaag tcccaagtga ataccggatt cttcctgagc
     9961 ctgcaggact acaacggcga cacacagaag aagctgcacc tgagcggagt gggaatcgcc
    10021 ctgctgatct gcctgttcct ggacaagcag tacatcaaca tctttctgag caggctgccc
    10081 atcttctcca gctacaatgc ccagagcgag gaacggcgga tcatcatcag atccttcggc
    10141 atcaacagca tcaagctgcc caaggaccgg atccacagcg agaagtccaa caagagcgtg
    10201 gccatggata tgctcaacga agtgaagcgg tgccccgacg agctgttcac aacactgtct
    10261 gccgagaagc agtcccggtt cagaatcatc agcgacgacc acaatgaagt gctgatgaag
    10321 cggagcagcg acagattcgt gcctctgctg ctgcagtata tcgattacgg caagctgttc
    10381 gaccacatca ggttccacgt gaacatgggc aagctgagat acctgctgaa ggccgacaag
    10441 acctgcatcg acggccagac cagagtcaga gtgatcgagc agcccctgaa cggcttcggc
    10501 agactggaag aggccgagac aatgcggaag caagagaacg gcaccttcgg caacagcggc
    10561 atccggatca gagacttcga gaacatgaag cgggacgacg ccaatcctgc caactatccc
    10621 tacatcgtgg acacctacac acactacatc ctggaaaaca acaaggtcga gatgtttatc
    10681 aacgacaaag aggacagcgc cccactgctg cccgtgatcg aggatgatag atacgtggtc
    10741 aagacaatcc ccagctgccg gatgagcacc ctggaaattc cagccatggc cttccacatg
    10801 tttctgttcg gcagcaagaa aaccgagaag ctgatcgtgg acgtgcacaa ccggtacaag
    10861 agactgttcc aggccatgca gaaagaagaa gtgaccgccg agaatatcgc cagcttcgga
    10921 atcgccgaga gcgacctgcc tcagaagatc ctggatctga tcagcggcaa tgcccacggc
    10981 aaggatgtgg acgccttcat cagactgacc gtggacgaca tgctgaccga caccgagcgg
    11041 agaatcaaga g
//
