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pBABE-neo largeTgenomic vector (V017934)

Basic Vector Information

Vector Name:
pBABE-neo largeTgenomic
Antibiotic Resistance:
Ampicillin
Length:
6085 bp
Type:
Protein expression, Immortalization
Host:
Mammalian cells, Retrovirus
Selection Marker:
Neo/G418
Promoter:
MMLV
5' Primer:
pBABE-5
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pBABE-neo largeTgenomic vector Map

Copy Sequence View Map
pBABE-neo largeTgenomic6085 bp30060090012001500180021002400270030003300360039004200450048005100540057006000MMLV Ψgag (truncated)small t antigenSV40 NLSSV40 promoterNeoR/KanRLTRAmpRAmpR promoter

pBABE-neo largeTgenomic vector Sequence

LOCUS       .                       6085 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    542..741
                     /label="MMLV Ψ"
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             942..1358
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     CDS             1425..1946
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             2146..2166
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     promoter        2229..2558
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2915..3706
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     LTR             4391..4982
                     /label="LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     CDS             complement(5100..5957)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(5958..6062)
                     /label="AmpR promoter"

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