LOCUS       .                       6085 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    542..741
                     /label="MMLV Ψ"
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             942..1358
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     CDS             1425..1946
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             2146..2166
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     promoter        2229..2558
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2915..3706
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     LTR             4391..4982
                     /label="LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     CDS             complement(5100..5957)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(5958..6062)
                     /label="AmpR promoter"
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccga agtagtgagg
     1381 aggctttttt ggaggcctag gcttttgcaa aaagctttgc aaagatggat aaagttttaa
     1441 acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt gcctggggga
     1501 atattcctct gatgagaaag gcatatttaa aaaaatgcaa ggagtttcat cctgataaag
     1561 gaggagatga agaaaaaatg aagaaaatga atactctgta caagaaaatg gaagatggag
     1621 taaaatatgc tcatcaacct gactttggag gcttctggga tgcaactgag gtatttgctt
     1681 cttccttaaa tcctggtgtt gatgcaatgt actgcaaaca atggcctgag tgtgcaaaga
     1741 aaatgtctgc taactgcata tgcttgctgt gcttactgag gatgaagcat gaaaatagaa
     1801 aattatacag gaaagatcca cttgtgtggg ttgattgcta ctgcttcgat tgctttagaa
     1861 tgtggtttgg acttgatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     1921 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     1981 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     2041 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     2101 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     2161 aaggtagaag ggatcccagt gtggtggtac gtaggaattc gccagcacag tggtcgaccc
     2221 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     2281 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     2341 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     2401 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     2461 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     2521 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttcacgct gccgcaagca
     2581 ctcagggcgc aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg
     2641 gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc
     2701 aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt
     2761 atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc
     2821 ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag
     2881 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     2941 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     3001 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     3061 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     3121 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     3181 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     3241 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     3301 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     3361 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     3421 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     3481 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     3541 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     3601 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     3661 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     3721 gggttcgcat cgatgcatcg atgcggcggg tgtggtggtt acgcgtaatc tgctgcttgc
     3781 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3841 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3901 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3961 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4021 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4081 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4141 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4201 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4261 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4321 gcctatggaa aaacgccagc aacgcatcga taaaataaaa gattttattt agtctccaga
     4381 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     4441 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     4501 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     4561 gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     4621 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc
     4681 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     4741 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     4801 cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact
     4861 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     4921 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     4981 cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta cattaaatgg
     5041 ccatagtgcg gccgcaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     5101 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5161 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5221 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5281 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5341 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5401 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5461 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5521 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5581 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5641 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5701 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5761 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5821 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5881 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5941 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     6001 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6061 cgcacatttc cccgaaaagt gccac
//
