pBARGPE1-MCS-3×FLAG-Neo vector (V017888)

LOCUS       .                       6167 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(9..74)
                     /label="3xFLAG"
                     /note="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
     primer_bind     77..93
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    1102..1242
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1428..2016)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2190..3047)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(3048..3152)
                     /label="AmpR promoter"
     promoter        3258..3359
                     /label="TRP1 promoter"
     promoter        3454..3813
                     /label="trpC promoter"
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             4189..4977
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     protein_bind    complement(5483..5516)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."