LOCUS       .                       6167 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(9..74)
                     /label="3xFLAG"
                     /note="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
     primer_bind     77..93
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    1102..1242
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1428..2016)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2190..3047)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(3048..3152)
                     /label="AmpR promoter"
     promoter        3258..3359
                     /label="TRP1 promoter"
     promoter        3454..3813
                     /label="trpC promoter"
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             4189..4977
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     protein_bind    complement(5483..5516)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggtacctact tgtcatcgtc atccttgtag tcgatgtcat gatctttata atcaccgtca
       61 tggtctttgt agtcctcgag gtcgacggta tcgataagct tgatatcgaa ttcaagcacc
      121 atggatcctc tagagtcgag gtcgacggta tcgataagct tgattaaagg ttcttggatg
      181 ggaagatgaa tatactgaag atgggaaaag aaagagaaaa gaaaagagca gctggtgggg
      241 agagcaggaa aatatggcaa caaatgttgg actgacgcaa cgaccttgtc aaccccgccg
      301 acacaccggg cggacagacg gggcaaagct gcctaccagg gactgaggga cctcagcagg
      361 tcgagtgcag agcaccggat gggtcgactg ccagcttgtg ttcccggtct gcgccgctgg
      421 ccagctcctg agcggccttt ccggtttcat acaccgggca aagcaggaga ggcacgatat
      481 ttggacgccc tacagatgcc ggatgggcca attagggagc ttacgcgccg ggtactcgct
      541 ctacctactt cggagaaggt actatctcgt gaatctttta ccagatcgga agcaattgga
      601 cttctgtacc taggttaatg gcatgctatt tcgccgacgg ctatacaccc ctggcttcac
      661 attctccttc gcttactgcc ggtgattcga tgaagctcca tattctccga tgatgcaata
      721 gattcttggt caacgagggg cacaccagcc tttccacttc ggggcggagg ggcggccggt
      781 cccggattaa taatcatcca ctgcacctca gagccgccag agctgtctgg cgcagtggcg
      841 cttattactc agcccttctc tctgcgtccg tccgtctctc cgcatgcttt gcatacttct
      901 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagagatct
      961 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     1021 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     1081 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     1141 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     1201 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     1261 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1321 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1381 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1441 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1501 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1561 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1621 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1681 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1741 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1801 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1861 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1921 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1981 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2041 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2101 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2161 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2221 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2281 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2341 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2401 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2461 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2521 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2581 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2641 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2701 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2761 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2821 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2881 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2941 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3001 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3061 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3121 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3181 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3241 tttcgtcttc aagaattaat tcggtcgaaa aaagaaaagg agagggccaa gagggagggc
     3301 attggtgact attgagcacg tgagtatacg tgattaagca cacaaaggca gcttggagta
     3361 tgtctgttat taatttcaca ggtagttctg gtccattggt gaaagtttgc ggcttgcaga
     3421 gcacagaggc cgcagaatgt gctctagact cgacagaaga tgatattgaa ggagcacttt
     3481 ttgggcttgg ctggagctag tggaggtcaa caatgaatgc ctattttggt ttagtcgtcc
     3541 aggcggtgag cacaaaattt gtgtcgtttg acaagatggt tcatttaggc aactggtcag
     3601 atcagcccca cttgtagcag tagcggcggc gctcgaagtg tgactcttat tagcagacag
     3661 gaacgaggac attattatca tctgctgctt ggtgcacgat aacttggtgc gtttgtcaag
     3721 caaggtaagt gaacgacccg gtcatacctt cttaagttcg cccttcctcc ctttatttca
     3781 gattcaatct gacttaccta ttctacccaa gcaaagcttc gattaggaag taaccatgaa
     3841 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     3901 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     3961 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     4021 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     4081 gtttagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     4141 cctgcctgaa accgaactgc ccgctgttct acaaccggtg ccaccatgat tgaacaagat
     4201 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     4261 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     4321 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     4381 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     4441 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     4501 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     4561 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     4621 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     4681 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga tgatctcgtc
     4741 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     4801 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     4861 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     4921 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     4981 tccgtcgacc tgcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     5041 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     5101 ttaacatgta atgcatagta ccgagaaact agtggatcta aacataaaat ctgtaaaata
     5161 acaagatgta aagataatgc taaatcattt ggctttttga ttgattgtac aggaaaatat
     5221 acatcgcagg gggttgactt ttaccatttc accgcaatgg aatcaaactt gttgaagaga
     5281 atgttcacag gcgcatacgc tacaatgacc cgattcttgc tagccttttc tcggtcttgc
     5341 aaacaaccgc cgcggccggt cgagggacct aataacttcg tatagcatac attatacgaa
     5401 gttatattaa gggttccgga tcgcggccgc tcgacctgca gccaagctag cttggctgga
     5461 cgtaaactcc tcttcagacc taataacttc gtatagcata cattatacga agttatatta
     5521 agggttattg aatatgatcg gaattggtcg atcaggtatt gctgttatct gatgagtata
     5581 cgttgtcctg gccacggcag aagcacgctt atcgctccaa tttcccacaa cattagtcaa
     5641 ctccgttagg cccttcattg aaagaaatga ggtcatcaaa tgtcttccaa tgtgagattt
     5701 tgggccattt tttatagcaa agattgaata aggcgcattt ttcttcaaag cttgcaaatt
     5761 aaagccttcg agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg
     5821 tacacgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
     5881 ataaaaaccc atctcataaa taacgtcatg cattgcagat gagctgtatc tggaagaggt
     5941 aaacccgaaa cgcgttttat tcttgttgac atggagctat taaatcacta gaaggcactc
     6001 tttgctgctt ggacaaatga acgtatctta tcgagatcct gaacaccatt tgtctcaact
     6061 ccggagctga catcgacacc aacgatctta tatccagatt cgtcaagctg tttgatgatt
     6121 tcagtaacgt taagtggatc tcggtgacgg gcaggaccgg acggggc
//
