Basic Vector Information
- Vector Name:
- pCBH-Cas13X.1-U6-MCS-CMV-EGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7835 bp
- Type:
- Gene-editing vector
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- U6
- 5' Primer:
- CMV-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pCBH-Cas13X.1-U6-MCS-CMV-EGFP vector Map
pCBH-Cas13X.1-U6-MCS-CMV-EGFP vector Sequence
LOCUS . 7835 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
enhancer 396..681
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 683..960
/label="chicken β-actin promoter"
CDS 1210..1230
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 3553..3573
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 3583..3717
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 3724..3964
/label="U6 promoter"
/note="RNA polymerase III promoter for human U6 snRNA"
enhancer 4114..4417
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 4418..4621
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 4656..5372
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
polyA_signal 5392..5616
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
promoter 6038..6142
/label="AmpR promoter"
CDS 6143..7000
/label="AmpR"
/note="β-lactamase"
rep_origin 7174..7762
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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