LOCUS       .                       7835 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        396..681
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer;
                     contains an 18-bp deletion relative to the standard CMV
                     enhancer"
     promoter        683..960
                     /label="chicken β-actin promoter"
     CDS             1210..1230
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             3553..3573
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    3583..3717
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        3724..3964
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     enhancer        4114..4417
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4418..4621
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             4656..5372
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     polyA_signal    5392..5616
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6038..6142
                     /label="AmpR promoter"
     CDS             6143..7000
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      7174..7762
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tatcccctga ttctctcgag ttaaccctag aaagataatc atattgtgac gtacgttaaa
       61 gataatcatg cgtaaaattg acgcatgtgt tttatcgatc tgtatatcga ggtttattta
      121 ttaatttgaa tagatattaa gttttattat atttacactt acatactaat aataaattca
      181 acaaacaatt tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt
      241 ctataaagta acaaaacttt taaacattct ctcttttaca aaaataaact tattttgtac
      301 tttaaaaaca gtcatgttgt attataaaat aagtaattag cttaacttat acataataga
      361 aacaaattat acttattaat taatctagag gtacccgtta cataacttac ggtaaatggc
      421 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg
      481 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      541 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      601 tggcattgtg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      661 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      721 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      781 atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
      841 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      901 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      961 ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
     1021 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     1081 tccgggctgt aattagctga gcaagaggta agggtttaag ggatggttgg ttggtggggt
     1141 attaatgttt aattacctgg agcacctgcc tgaaatcact ttttttcagg ttggaccggt
     1201 gccaccatgc ccaagaagaa gcggaaggtg gcccaggtga gcaagcagac ctccaagaag
     1261 agggagctga gcatcgacga gtaccagggc gcccggaagt ggtgcttcac cattgccttc
     1321 aacaaggccc tggtgaaccg ggacaagaac gacggcctgt tcgtggaaag cctgctgaga
     1381 cacgagaagt acagcaagca cgactggtac gacgaagata cccgggccct gatcaagtgc
     1441 agcacccagg ccgccaacgc caaggctgaa gccctgcgga actacttcag tcactaccgg
     1501 catagccctg gctgcctgac cttcaccgcc gaggacgaac tgcggaccat catggagaga
     1561 gcctatgagc gggccatctt cgagtgcaga agaagagaga cagaggtgat catcgagttt
     1621 cccagcctgt tcgagggcga ccggatcacc accgccggcg tggtgttttt cgtgagcttt
     1681 ttcgtggaaa gaagagtgct ggatcggctg tatggagccg tgtccggcct gaagaagaat
     1741 gagggacagt acaagctgac ccggaaggcc ctgagcatgt actgcctgaa ggacagcaga
     1801 ttcaccaagg cctgggataa gcgggtgctg ctgttcagag acatcctggc ccagctggga
     1861 agaatccccg ccgaggccta cgagtactac cacggcgagc agggtgataa gaagagagct
     1921 aacgacaatg agggcacaaa tcccaagcgg cacaaggaca agttcatcga atttgcactg
     1981 cactacctgg aagcccagca cagcgagatc tgcttcggca gacgccacat cgtgcgggaa
     2041 gaggccggcg ccggcgatga gcacaagaag caccggacca agggaaaggt ggtggtggac
     2101 ttcagcaaga aggacgagga ccagagctac tatatctcca agaacaacgt gatcgtgcgg
     2161 atcgacaaga acgccggccc tagaagctac cggatgggcc tgaacgagct gaagtacctc
     2221 gtgctgctga gcctgcaggg gaagggcgac gatgccatcg ccaagctgta cagatacaga
     2281 cagcacgtgg agaacatcct ggatgtggtg aaggtgaccg ataaggataa ccacgtgttc
     2341 ctgccccgct tcgtgctgga gcagcacggc atcggcagaa aggccttcaa gcagcggatc
     2401 gatggacggg tgaagcacgt gcggggcgtg tgggagaaga agaaggccgc caccaatgaa
     2461 atgaccctgc acgagaaggc cagagacatc ctgcagtacg tgaacgaaaa ctgcacccgg
     2521 tccttcaacc ctggcgaata caacagactg ctggtgtgcc tggtgggcaa ggacgtggag
     2581 aactttcagg ccggcctgaa gcggctgcag ctggccgaaa ggatcgatgg ccgggtgtac
     2641 tccatcttcg cccagaccag caccatcaat gagatgcacc aggtggtgtg cgaccagatc
     2701 ctgaaccggc tgtgcagaat cggcgaccag aagctgtacg attacgtggg actgggcaag
     2761 aaggacgaaa tcgactacaa gcagaaggtg gcctggttca aggagcacat cagcatccgg
     2821 agaggattcc tgagaaagaa gttctggtac gatagcaaga agggattcgc aaagctggtg
     2881 gaggaacacc tggagtccgg cggcggccag cgcgacgtgg gcctggacaa gaagtactac
     2941 cacatcgacg ccatcggcag attcgagggc gccaaccccg ccctgtacga gaccctggcc
     3001 agagatcggc tgtgcctcat gatggcccag tacttcctgg gcagcgtgag aaaggaactg
     3061 ggcaacaaga ttgtgtggag caacgacagc atcgaactgc ctgtggaagg ctctgtggga
     3121 aatgagaaga gcatcgtgtt ctccgtgtct gactacggca agctgtacgt gctggacgat
     3181 gccgaattcc tgggccggat ctgcgaatac ttcatgcccc acgaaaaggg caagatccgg
     3241 taccacacag tgtacgaaaa gggctttaga gcatacaacg acctgcagaa gaagtgcgtg
     3301 gaggccgtgc tggctttcga agagaaggtg gtgaaggcca agaagatgag cgagaaggaa
     3361 ggcgcccact acatcgactt ccgggagatc ctggcccaga ccatgtgcaa ggaggccgaa
     3421 aagaccgcag tgaacaaggt gagacgcgcc ttcttccacc accacctgaa gttcgtgatt
     3481 gacgagttcg gcctgttcag cgacgtgatg aagaagtacg gcatcgagaa ggaatggaag
     3541 ttccctgtca agcccaagaa gaagcggaag gtgtagaagc ttaacttgtt tattgcagct
     3601 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3661 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcact
     3721 agtgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     3781 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     3841 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     3901 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     3961 ggacgaaaca ccggggtctt cgatattcaa gcgtcggaag acctgctgga gcagcccccg
     4021 atttgtgggg tgattacagc tttttttacg cgttagttat taatagtaat caattacggg
     4081 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     4141 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     4201 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     4261 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     4321 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     4381 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     4441 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     4501 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     4561 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     4621 tggtttagtg aaccgtcaga tccgctagcg ccaccatggt gagcaagggc gaggagctgt
     4681 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4741 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     4801 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     4861 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4921 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4981 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     5041 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     5101 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     5161 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     5221 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     5281 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     5341 ggatcactct cggcatggac gagctgtaca agtaactctt ggcccggatc cctgtgcctt
     5401 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     5461 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     5521 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaaaaca
     5581 atagcaggca tgctggggat gcggtgggct ctatgggtcg acggcgcgcc ctagacgaag
     5641 cagctccagc ctacacaatc gctcaagacg tgtaatgctt ttattatata ttagtcacga
     5701 tatctataac aagaaaatat atatataata agttatcacg taagtagaac atgaaataac
     5761 aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt
     5821 ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc
     5881 ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct
     5941 atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt
     6001 tctagggtta aggatccgca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6061 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6301 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6361 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     6421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     6541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     6601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     6661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     6721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     6781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     6841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7021 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7081 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7141 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7201 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7261 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7321 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7381 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7441 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7501 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7561 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7621 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7681 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7741 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7801 ttgctggcct tttgctcaca tgttctttcc tgcgt
//
