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pAAV-CAG-Rab4(fly)-S22N-mNeonGreen vector (V017819)

Basic Vector Information

Vector Name:
pAAV-CAG-Rab4(fly)-S22N-mNeonGreen
Antibiotic Resistance:
Ampicillin
Length:
6542 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells, Adeno-associated virus
Promoter:
CAG
5' Primer:
pcaggs-F
Growth Strain(s):
Stbl3
Growth Temperature:
37℃

pAAV-CAG-Rab4(fly)-S22N-mNeonGreen vector Map

Copy Sequence View Map
pAAV-CAG-Rab4(fly)-S22N-mNeonGreen6542 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300CMV enhancerchicken β-actin promotermNeonGreenWPREhGH poly(A) signalAAV2 ITRf1 oriAmpR promoterAmpRori

pAAV-CAG-Rab4(fly)-S22N-mNeonGreen vector Sequence

LOCUS       .                       6542 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        210..513
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        515..792
                     /label="chicken β-actin promoter"
     CDS             1934..2641
                     /label="mNeonGreen"
                     /note="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
     misc_feature    2669..3257
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    3289..3765
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     repeat_region   3805..3945
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     rep_origin      4020..4475
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4757..4861
                     /label="AmpR promoter"
     CDS             4862..5719
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      5893..6481
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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