LOCUS       .                       6542 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        210..513
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        515..792
                     /label="chicken β-actin promoter"
     CDS             1934..2641
                     /label="mNeonGreen"
                     /note="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
     misc_feature    2669..3257
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    3289..3765
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     repeat_region   3805..3945
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     rep_origin      4020..4475
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4757..4861
                     /label="AmpR promoter"
     CDS             4862..5719
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      5893..6481
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaacact agttattaat agtaatcaat tacggggtca
      181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      481 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
      541 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
      601 ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      661 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
      721 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
      781 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
      841 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
      901 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
      961 tggctgcgtg aaagccttga ggggctccgg gagcgccggc aggaaggaaa tgggcgggga
     1021 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1081 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1141 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1201 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgcggcccaa
     1261 cggtaccgcc accatgtcgg aaacatacga ttacctgttc aagttcctga taattggcag
     1321 tgctggcagc ggtaagaatt gtctgttgca tcacttcatc gagagcaagt tcaaagacga
     1381 cagttcgcac accatcggag tggagttcgg ctcgcggatt gtgaacgtgg gcggcaagtc
     1441 ggtaaagcta cagatatggg acacagccgg tcaggagaga ttccgatcgg tgacaagatc
     1501 gtactatcgc ggagcagccg gagctctcct ggtctacgat gccacttcgc gggactcttt
     1561 caatgctttg acaaactggc tgaatgatgc tcgcactctg gccagcccaa atattgtgat
     1621 ccttttggtg ggcaataaaa aggacttgga ggaggcgcgt gatgtgacct ttctggaagc
     1681 aagcaccttt gcccaagaga acgagcttat cttcctggag acgagcgcca agacggggga
     1741 gaatgttgaa gaggcctttc tcaagtgctc aaagaccata ctggccaaaa ttgagaccgg
     1801 agagctggat cccgagcgca tcggcagcgg tattcagtac ggcggagctg ctctccgtaa
     1861 cttacagaca cgacagcgca gcatcaataa gccggattgc acctgtcgcg ttggaggtgg
     1921 tggatcagcc accatggtga gcaagggcga ggaggataac atggcctctc tcccagcgac
     1981 acatgagtta cacatctttg gctccatcaa cggtgtggac tttgacatgg tgggtcaggg
     2041 caccggcaat ccaaatgatg gttatgagga gttaaacctg aagtccacca agggtgacct
     2101 ccagttctcc ccctggattc tggtccctca tatcgggtat ggcttccatc agtacctgcc
     2161 ctaccctgac gggatgtcgc ctttccaggc cgccatggta gatggctccg gataccaagt
     2221 ccatcgcaca atgcagtttg aagatggtgc ctcccttact gttaactacc gctacaccta
     2281 cgagggaagc cacatcaaag gagaggccca ggtgaagggg actggtttcc ctgctgacgg
     2341 tcctgtgatg accaactcgc tgaccgctgc ggactggtgc aggtcgaaga agacttaccc
     2401 caacgacaaa accatcatca gtacctttaa gtggagttac accactggaa atggcaagcg
     2461 ctaccggagc actgcgcgga ccacctacac ctttgccaag ccaatggcgg ctaactatct
     2521 gaagaaccag ccgatgtacg tgttccgtaa gacggagctc aagcactcca agaccgagct
     2581 caacttcaag gagtggcaaa aggcctttac cgatgtgatg ggcatggacg agctgtacaa
     2641 gtgagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     2701 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     2761 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     2821 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     2881 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     2941 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     3001 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     3061 gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac
     3121 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     3181 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     3241 ttgggccgcc tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc
     3301 tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc
     3361 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt
     3421 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc
     3481 tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc
     3541 tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag
     3601 gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata
     3661 ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa
     3721 ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta
     3781 accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     3841 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     3901 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     3961 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     4021 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4081 tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4141 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4201 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     4261 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4321 actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata
     4381 agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4441 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     4501 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4561 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4621 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4681 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4741 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4801 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4861 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4921 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4981 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5041 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5101 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5161 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5221 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5281 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5341 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5401 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5461 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5521 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5581 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5641 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5701 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5761 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5821 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5881 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5941 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6001 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6061 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6121 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6181 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6241 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6301 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6361 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6421 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6481 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6541 gt
//
