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pAOX1-α-factor-ENA(apostichopus-opt-yeast)-X2-6×His vector (V017766)

Basic Vector Information

Vector Name:
pAOX1-α-factor-ENA(apostichopus-opt-yeast)-X2-6×His
Antibiotic Resistance:
Ampicillin
Length:
10142 bp
Type:
Protein expression
Replication origin:
ori
Host:
Yeast
Selection Marker:
HIS4
Promoter:
AOX
3' Primer:
3'AOX
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pAOX1-α-factor-ENA(apostichopus-opt-yeast)-X2-6×His vector Map

Copy Sequence View Map
pAOX1-α-factor-ENA(apostichopus-opt-yeast)-X2-6×His10142 bp50010001500200025003000350040004500500055006000650070007500800085009000950010000AOX1 promoterα-factor secretion signal6xHisAOX1 terminatorKanRAOX1 3' fragmentbomoriAmpRAmpR promoter

pAOX1-α-factor-ENA(apostichopus-opt-yeast)-X2-6×His vector Sequence

LOCUS       .                      10142 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        2..937
                     /label="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             949..1215
                     /label="α-factor secretion signal"
                     /note="N-terminal secretion signal from S. cerevisiae
                     alpha-factor"
     CDS             2146..2163
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      2187..2433
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     CDS             complement(5797..6609)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    6988..7744
                     /label="AOX1 3' fragment"
                     /note="region downstream of Pichia pastoris AOX1 gene"
     misc_feature    7887..8027
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8213..8801)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8975..9832)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(9833..9937)
                     /label="AmpR promoter"

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