LOCUS       .                      10142 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        2..937
                     /label="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             949..1215
                     /label="α-factor secretion signal"
                     /note="N-terminal secretion signal from S. cerevisiae
                     alpha-factor"
     CDS             2146..2163
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      2187..2433
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     CDS             complement(5797..6609)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    6988..7744
                     /label="AOX1 3' fragment"
                     /note="region downstream of Pichia pastoris AOX1 gene"
     misc_feature    7887..8027
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8213..8801)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8975..9832)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(9833..9937)
                     /label="AmpR promoter"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct
      961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact
     1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta
     1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg
     1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa
     1201 agagaggctg aagcttacgt agaattcatg agaacatttg gtagtatcga atttaatatg
     1261 agaagatgtt ctgaaggtga agaaggcgaa tcgagaaagt caaatagacc cagtttattt
     1321 tccaattaca cttgttgtac ccaagctgca tcagatggtt ccttttcttg gactttgtca
     1381 aagtctaaga gaatggctaa acaatctaac tctgggcttg atatgtattc atctcaaagt
     1441 cccagacact ctccaacacc accatcccaa tcacaacaag ttaacaataa ttcttttgac
     1501 caagatgcaa gctctggttc atcttcaggt tctggtactg ctagtagtaa ttccacccca
     1561 agatctccaa gcagatcaaa atccccaaga ccacatagag ttgaaggttc cagtagattt
     1621 cctgaagcaa aaatggaaac ttttcagtcc ttcaatgctg catataacgc tggcccagtt
     1681 tacatctctg aaggtttggg ttctccagtt ccaggtggtt ctacttcccc aaattcatct
     1741 ggtaatcatg ctagaagtaa ttccccagtc cattccagat cttctggttc tagtcaaggt
     1801 agtggttcta atatcacttt aggtcacggt actcatccag gtgctcatcc accaggtcat
     1861 ccattgcatc atagagctcc agattttgac tctatggacg tttcttcaca attgagagaa
     1921 ttgcaaagag aaaacacaat gtatagacaa gaactagatg ctagagacgc taaattgtcc
     1981 tcctcaatga attctattaa gacattctgg tcaccagaat tgaaaaaaga aagggcttta
     2041 agaaaggaag aggctggtaa gattcaggct ttgaaagaac aatgtaaaaa agccgttgaa
     2101 gaaaatcagg tctcattgtt gtttgatttg tcaccatgcg gccgccacca tcaccatcac
     2161 cattaaccgg tcttgctaga ttctaatcaa gaggatgtca gaatgccatt tgcctgagag
     2221 atgcaggctt catttttgat acttttttat ttgtaaccta tatagtatag gatttttttt
     2281 gtcattttgt ttcttctcgt acgagcttgc tcctgatcag cctatctcgc agctgatgaa
     2341 tatcttgtgg taggggtttg ggaaaatcat tcgagtttga tgtttttctt ggtatttccc
     2401 actcctcttc agagtacaga agattaagtg agacgttcgt ttgtgcaagc ttatcgataa
     2461 gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa
     2521 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg
     2581 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc
     2641 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt
     2701 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg
     2761 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatct atcgaatcta
     2821 aatgtaagtt aaaatctcta aataattaaa taagtcccag tttctccata cgaaccttaa
     2881 cagcattgcg gtgagcatct agaccttcaa cagcagccag atccatcact gcttggccaa
     2941 tatgtttcag tccctcagga gttacgtctt gtgaagtgat gaacttctgg aaggttgcag
     3001 tgttaactcc gctgtattga cgggcatatc cgtacgttgg caaagtgtgg ttggtaccgg
     3061 aggagtaatc tccacaactc tctggagagt aggcaccaac aaacacagat ccagcgtgtt
     3121 gtacttgatc aacataagaa gaagcattct cgatttgcag gatcaagtgt tcaggagcgt
     3181 actgattgga catttccaaa gcctgctcgt aggttgcaac cgatagggtt gtagagtgtg
     3241 caatacactt gcgtacaatt tcaacccttg gcaactgcac agcttggttg tgaacagcat
     3301 cttcaattct ggcaagctcc ttgtctgtca tatcgacagc caacagaatc acctgggaat
     3361 caataccatg ttcagcttga gacagaaggt ctgaggcaac gaaatctgga tcagcgtatt
     3421 tatcagcaat aactagaact tcagaaggcc cagcaggcat gtcaatacta cacagggctg
     3481 atgtgtcatt ttgaaccatc atcttggcag cagtaacgaa ctggtttcct ggaccaaata
     3541 ttttgtcaca cttaggaaca gtttctgttc cgtaagccat agcagctact gcctgggcgc
     3601 ctcctgctag cacgatacac ttagcaccaa ccttgtgggc aacgtagatg acttctgggg
     3661 taagggtacc atccttctta ggtggagatg caaaaacaat ttctttgcaa ccagcaactt
     3721 tggcaggaac acccagcatc agggaagtgg aaggcagaat tgcggttcca ccaggaatat
     3781 agaggccaac tttctcaata ggtcttgcaa aacgagagca gactacacca gggcaagtct
     3841 caacttgcaa cgtctccgtt agttgagctt catggaattt cctgacgtta tctatagaga
     3901 gatcaatggc tctcttaacg ttatctggca attgcataag ttcctctggg aaaggagctt
     3961 ctaacacagg tgtcttcaaa gcgactccat caaacttggc agttagttct aaaagggctt
     4021 tgtcaccatt ttgacgaaca ttgtcgacaa ttggtttgac taattccata atctgttccg
     4081 ttttctggat aggacgacga agggcatctt caatttcttg tgaggaggcc ttagaaacgt
     4141 caattttgca caattcaata cgaccttcag aagggacttc tttaggtttg gattcttctt
     4201 taggttgttc cttggtgtat cctggcttgg catctccttt ccttctagtg acctttaggg
     4261 acttcatatc caggtttctc tccacctcgt ccaacgtcac accgtacttg gcacatctaa
     4321 ctaatgcaaa ataaaataag tcagcacatt cccaggctat atcttccttg gatttagctt
     4381 ctgcaagttc atcagcttcc tccctaattt tagcgttcaa caaaacttcg tcgtcaaata
     4441 accgtttggt ataagaacct tctggagcat tgctcttacg atcccacaag gtggcttcca
     4501 tggctctaag accctttgat tggccaaaac aggaagtgcg ttccaagtga cagaaaccaa
     4561 cacctgtttg ttcaaccaca aatttcaagc agtctccatc acaatccaat tcgataccca
     4621 gcaacttttg agttgctcca gatgtagcac ctttatacca caaaccgtga cgacgagatt
     4681 ggtagactcc agtttgtgtc cttatagcct ccggaataga ctttttggac gagtacacca
     4741 ggcccaacga gtaattagaa gagtcagcca ccaaagtagt gaatagacca tcggggcggt
     4801 cagtagtcaa agacgccaac aaaatttcac tgacagggaa ctttttgaca tcttcagaaa
     4861 gttcgtattc agtagtcaat tgccgagcat caataatggg gattatacca gaagcaacag
     4921 tggaagtcac atctaccaac tttgcggtct cagaaaaagc ataaacagtt ctactaccgc
     4981 cattagtgaa acttttcaaa tcgcccagtg gagaagaaaa aggcacagcg atactagcat
     5041 tagcgggcaa ggatgcaact ttatcaacca gggtcctata gataacccta gcgcctggga
     5101 tcatcctttg gacaactctt tctgccaaat ctaggtccaa aatcacttca ttgataccat
     5161 tattgtacaa cttgagcaag ttgtcgatca gctcctcaaa ttggtcctct gtaacggatg
     5221 actcaacttg cacattaact tgaagctcag tcgattgagt gaacttgatc aggttgtgca
     5281 gctggtcagc agcataggga aacacggctt ttcctaccaa actcaaggaa ttatcaaact
     5341 ctgcaacact tgcgtatgca ggtagcaagg gaaatgtcat acttgaagtc ggacagtgag
     5401 tgtagtcttg agaaattctg aagccgtatt tttattatca gtgagtcagt catcaggaga
     5461 tcctctacgc cggacgcatc gtggccgacc tgcagggggg gggggggcgc tgaggtctgc
     5521 ctcgtgaaga aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga
     5581 aagtgaggga gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga
     5641 acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca
     5701 actcagcaaa agttcgattt attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct
     5761 ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg
     5821 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     5881 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     5941 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     6001 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt
     6061 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     6121 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
     6181 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     6241 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     6301 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     6361 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     6421 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     6481 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     6541 taaatcagca tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat
     6601 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
     6661 tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttc
     6721 cccccccccc ctgcaggtcg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     6781 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     6841 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     6901 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
     6961 aatgcaggag tcgcataagg gagagcgtcg agtatctatg attggaagta tgggaatggt
     7021 gatacccgca ttcttcagtg tcttgaggtc tcctatcaga ttatgcccaa ctaaagcaac
     7081 cggaggagga gatttcatgg taaatttctc tgacttttgg tcatcagtag actcgaactg
     7141 tgagactatc tcggttatga cagcagaaat gtccttcttg gagacagtaa atgaagtccc
     7201 accaataaag aaatccttgt tatcaggaac aaacttcttg tttcgaactt tttcggtgcc
     7261 ttgaactata aaatgtagag tggatatgtc gggtaggaat ggagcgggca aatgcttacc
     7321 ttctggacct tcaagaggta tgtagggttt gtagatactg atgccaactt cagtgacaac
     7381 gttgctattt cgttcaaacc attccgaatc cagagaaatc aaagttgttt gtctactatt
     7441 gatccaagcc agtgcggtct tgaaactgac aatagtgtgc tcgtgttttg aggtcatctt
     7501 tgtatgaata aatctagtct ttgatctaaa taatcttgac gagccaaggc gataaatacc
     7561 caaatctaaa actcttttaa aacgttaaaa ggacaagtat gtctgcctgt attaaacccc
     7621 aaatcagctc gtagtctgat cctcatcaac ttgaggggca ctatcttgtt ttagagaaat
     7681 ttgcggagat gcgatatcga gaaaaaggta cgctgatttt aaacgtgaaa tttatctcaa
     7741 gatctctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     7801 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     7861 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     7921 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     7981 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     8041 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8101 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8161 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8221 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8281 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8341 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8401 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8461 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8521 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8581 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8641 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8701 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8761 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8821 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8881 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8941 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9001 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9061 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9121 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9181 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9241 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
     9301 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9361 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9421 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9481 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9541 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     9601 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9661 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9721 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9781 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9841 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9901 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9961 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    10021 ggccctttcg tcttcaagaa ttaattctca tgtttgacag cttatcatcg ataagctgac
    10081 tcatgttggt attgtgaaat agacgcagat cgggaacact gaaaaataac agttattatt
    10141 cg
//
