pHBV-Core promoter-Fluc vector (V017750)

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Basic Vector Information

Vector Name:: pHBV-Core promoter-Fluc

Antibiotic Resistance:: Ampicillin

Length:: 5009 bp

Type:: Promoter probe vector

Replication origin:: ori

Host:: Mammalian cells

5' Primer:: RVP3

Growth Strain(s):: DH5a

Growth Temperature:: 37℃

pHBV-Core promoter-Fluc vector Map

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pHBV-Core promoter-Fluc vector Sequence

LOCUS       .                       5009 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             279..1928
                     /label="luciferase"
                     /note="firefly luciferase"
     polyA_signal    complement(1972..2093)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2512..3100)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3274..4131)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(4132..4236)
                     /label="AmpR promoter"
     rep_origin      4263..4718
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4849..4897
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    4911..5002
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human α2 globin gene"

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