LOCUS       .                       5009 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             279..1928
                     /label="luciferase"
                     /note="firefly luciferase"
     polyA_signal    complement(1972..2093)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2512..3100)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3274..4131)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(4132..4236)
                     /label="AmpR promoter"
     rep_origin      4263..4718
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4849..4897
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    4911..5002
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human α2 globin gene"
ORIGIN
        1 ggtaccaacc accgtgaacg cccgccaggt cttgcccaag gtcttatata agaggactct
       61 tggactctca gtaatgtcaa cgaccgacct tgaggcatac ttcaaagact gtgtgtttaa
      121 agactgggag gagttggggg aggagattag gttaatgatc tttgtactag gaggctgtag
      181 gcataaattg gtctgttcac cagcaccatg caactttttc acctctgcct aatcatctct
      241 tgtaagcttg gcattccggt actgttggta aagccaccat ggaagacgcc aaaaacataa
      301 agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata
      361 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
      421 aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga
      481 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
      541 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
      601 tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg
      661 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa
      721 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg
      781 tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata
      841 gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg
      901 tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg
      961 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
     1021 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
     1081 ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg
     1141 tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta
     1201 atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg
     1261 ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag
     1321 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat
     1381 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag
     1441 gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga
     1501 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
     1561 aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc
     1621 aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag
     1681 gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
     1741 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
     1801 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
     1861 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga
     1921 tcgccgtgta attctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca
     1981 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     2041 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     2101 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     2161 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga
     2221 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     2281 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc
     2341 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2401 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2461 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2521 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2581 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2641 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2701 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2761 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2821 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2881 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2941 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3001 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3061 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3121 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3181 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3241 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3301 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3361 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3421 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3481 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3541 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3601 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3661 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3721 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3781 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3841 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3901 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3961 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4021 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     4081 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     4141 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     4201 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     4261 tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4321 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4381 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4441 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     4501 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4561 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4621 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4681 taacgcgaat tttaacaaaa tattaacgtt tacaatttcc cattcgccat tcaggctgcg
     4741 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc ccaagctacc
     4801 atgataagta agtaatatta aggtacggga ggtacttgga gcggccgcaa taaaatatct
     4861 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct
     4921 ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt
     4981 gcaggtgcca gaacatttct ctatcgata
//
