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pFPV25.1-roGFP2-Orp1 vector (V017681)

Basic Vector Information

Vector Name:
pFPV25.1-roGFP2-Orp1
Antibiotic Resistance:
Ampicillin
Length:
5950 bp
Type:
Protein expression
Replication origin:
ori
Host:
E. coli
Copy Number:
Low
Promoter:
rpsM
5' Primer:
pEGFP-C-5'
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pFPV25.1-roGFP2-Orp1 vector Map

Copy Sequence View Map
pFPV25.1-roGFP2-Orp15950 bp60012001800240030003600420048005400oriAmpRAmpR promoterrpmJEGFPRSF1010 oriT

pFPV25.1-roGFP2-Orp1 vector Sequence

LOCUS       .                       5950 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      complement(182..770)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(944..1801)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(1802..1906)
                     /label="AmpR promoter"
     CDS             2235..2348
                     /gene="rpmJ"
                     /label="rpmJ"
                     /note="Large ribosomal subunit protein bL36A from
                     Escherichia coli (strain K12). Accession#: P0A7Q6"
     CDS             2640..3356
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     oriT            4561..4648
                     /label="RSF1010 oriT"
                     /note="origin of transfer of the broad-host-range plasmid
                     RSF1010 (Scholz et al., 1989)"

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