LOCUS       .                       5950 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      complement(182..770)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(944..1801)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(1802..1906)
                     /label="AmpR promoter"
     CDS             2235..2348
                     /gene="rpmJ"
                     /label="rpmJ"
                     /note="Large ribosomal subunit protein bL36A from
                     Escherichia coli (strain K12). Accession#: P0A7Q6"
     CDS             2640..3356
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     oriT            4561..4648
                     /label="RSF1010 oriT"
                     /note="origin of transfer of the broad-host-range plasmid
                     RSF1010 (Scholz et al., 1989)"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1981 gtatcacgag gccctttcgt cttcaagaat tcgagctcgg tacccgggga tcccggagtt
     2041 catgcgtgat gcaatgaaag tgccgttcta cttcggtggg acctcactgc ttatcgttgt
     2101 tgtcgtgatt atggacttta tggctcaagt gcaaactctg atgatgtcca gtcagtatga
     2161 gtctgcattg aagaaggcga acctgaaagg ctacggccgt taattggtcg cctgagaagt
     2221 tacggagagt aaaaatgaaa gttcgtgctt ccgtcaagaa attatgccgt aactgcaaaa
     2281 tcgttaagcg tgatggtgtc atccgtgtga tttgcagtgc cgagccgaag cataaacagc
     2341 gccaaggctg attttttcgc atatttttct tgcaaagttg ggttgagctg gctagattag
     2401 ccagccaatc ttttgtatgt ctgtacgttt ccatttgagt atcctgaaaa cgggcttttc
     2461 agcatggtac gtacatatta aatagtagga gtgcatagtg gcccgtatag caggcattaa
     2521 cattcctgat cagaaacacg ccgtgatcgc gttaacttcg atctacggtg tcggcaagac
     2581 ccgttctaaa gccatcctgg ctgcagcggg tatcgctgaa aatgttaaga tcctctagaa
     2641 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2701 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2761 gcaagctgac cctgaagttc atctccacca ccggcaagct gcccgtgccc tggcccaccc
     2821 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     2881 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     2941 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3001 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     3061 agctggagta caactacaac tgccacaacg tctatatcat ggccgacaag cagaagaacg
     3121 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     3181 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     3241 acctgagcac ctgctccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     3301 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagacta
     3361 gtggtggttc aggtggtggt ggttcaggtg gtggtggttc aggtggagga ggatcaggag
     3421 gaggaggatc aggaggagga ggatcaggag gagaattcga tatctcagaa ttctataagc
     3481 tagcacctgt tgacaagaaa ggccaaccat tccccttcga ccaattaaag ggaaaagtgg
     3541 tgcttatcgt taatgttgcc tccaaatgtg gattcactcc tcaatacaaa gaactagagg
     3601 ccttgtacaa acgttataag gacgaaggat ttaccatcat cgggttccca tgcaaccagt
     3661 ttggccacca agaacctggc tctgatgaag aaattgccca gttctgccaa ctgaactatg
     3721 gcgtgacttt ccccattatg aaaaaaattg acgttaatgg tggcaatgag gaccctgttt
     3781 acaagttttt gaagagccaa aaatccggta tgttgggctt gagaggtatc aaatggaatt
     3841 ttgaaaaatt cttagtcgat aaaaagggta aagtgtacga aagatactct tcactaacca
     3901 aaccttcttc gttgtccgaa accatcgaag aacttttgaa agaggtggaa tagaagcttt
     3961 aatgcggtag tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta
     4021 acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg
     4081 ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc atcgccagtc
     4141 actatggcgt gctgctagcg cttttagccg ctttagcggc ctttccccct acccgaaggg
     4201 tgggggcgcg tgtgcagccc cgcagggcct gtctcggtcg atcattcagc ccggctcatc
     4261 cttctggcgt ggcggcagac cgaacaaggc gcggtcgtgg tcgcgttcaa ggtacgcatc
     4321 cattgccgcc atgagccgat cctccggcca ctcgctgctg ttcaccttgg ccaaaatcat
     4381 ggcccccacc agcaccttgc gccttgtttc gttcttgcgc tcttgctgct gttcccttgc
     4441 ccgcacccgc tgaatttcgg cattgattcg cgctcgttgt tcttcgagct tggccagccg
     4501 atccgccgcc ttgttgctcc ccttaaccat cttgacaccc cattgttaat gtgctgtctc
     4561 gtaggctatc atggaggcac agcggcggca atcccgaccc tactttgtag gggagggggc
     4621 acttaccggt ttctcttcga gaaactggcc taacggccac ccttcgggcg gtgcgctctc
     4681 cgagggccat tgcatggagc cgaaaagcaa aagcaacagc gaggcagcat ggcgatttat
     4741 caccttacgg cgaaaaccgg cagcaggtcg ggcggccaat cggccagggc caaggccgac
     4801 tacatccagc gcgaaggcaa gtatgcccgc gacatggatg aagtcttgca cgccgaatcc
     4861 gggcacatgc cggagttcgt cgagcggccc gccgactact gggatgctgc cgacctgtat
     4921 gaacgcgcca atgggcggct gttcaaggag gtcgaatttg ccctgccggt cgagctgacc
     4981 ctcgaccagc agaaggcgct ggcgtccgag ttcgcccagc acctgaccgg tgccgagcgc
     5041 ctgccgtata cgctggccat ccatgccggt ggcggcgaga acccgcactg ccacctgatg
     5101 atctccgagc ggatcaatga cggcatcgag cggcccgccg ctcagtggtt caagcggtac
     5161 aacggcaaga ccccggagaa gggcggggca cagaagaccg aagcgctcaa gcccaaggca
     5221 tggcttgagc agacccgcga ggcatgggcc gaccatgcca accgggcatt agagcgggct
     5281 ggccacgacg cccgcattga ccacagaaca cttgaggcgc agggcatcga gcgcctgccc
     5341 ggtgttcacc tggggccgaa cgtggtggag atggaaggcc ggggcatccg caccgaccgg
     5401 gcagacgtgg ccctgaacat cgacaccgcc aacgcccaga tcatcgactt acaggaatac
     5461 cgggaggcaa tagaccatga acgcaatcga cagagtgaag aaatccagag gcatcaacga
     5521 gttagcggag cagatcgaac cgctggccca gagcatggcg acactggccg acgaagcccg
     5581 gcaggtcatg agccagaccc agcaggccag cgaggcgcag gcggcggagt ggctgaaagc
     5641 ccagcgccag acaggggcgg catgggtgga gctggccaaa gagttgcggg aggtagccgc
     5701 cgaggtgagc agcgccgcgc agagcgcccg gagcgcgtcg cgggggtggc actggaagct
     5761 atggctaacc gtgatgctgg cttccatgat gcctacggtg gtgctgctga tcgcatcgtt
     5821 gctcttgctc gacctgacgc cactgacaac cgaggacggc tcgatctggc tgcgcttggt
     5881 ggcccgatga agaacgacag gactttgcag gccataggcc gacagctcaa ggccatgggc
     5941 tgtgagcgct
//
