pADV-EF1a-MCS-Nluc vector (V017670)

LOCUS       .                       5148 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_signal     190..340
                     /label="Ad5 Ψ"
                     /note="packaging signal for adenovirus serotype 5"
     promoter        477..1655
                     /label="EF-1α promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
     CDS             1742..2254
                     /label="Nluc"
                     /note="NanoLuc® luciferase"
     polyA_signal    2271..2405
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2425..2458)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2480..2496)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2504..2520)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2528..2558)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2573..2594)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2882..3470)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3644..4501)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(4502..4606)
                     /label="AmpR promoter"
     repeat_region   5149..5250
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"