LOCUS       .                       5148 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_signal     190..340
                     /label="Ad5 Ψ"
                     /note="packaging signal for adenovirus serotype 5"
     promoter        477..1655
                     /label="EF-1α promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
     CDS             1742..2254
                     /label="Nluc"
                     /note="NanoLuc® luciferase"
     polyA_signal    2271..2405
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2425..2458)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2480..2496)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2504..2520)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2528..2558)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2573..2594)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2882..3470)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3644..4501)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(4502..4606)
                     /label="AmpR promoter"
     repeat_region   5149..5250
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
ORIGIN
        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
      421 gggtcaaagt tggcgtttta ttattatagt cagctctaga agaagagcat gcgtgaggct
      481 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
      541 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
      601 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
      661 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
      721 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
      781 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
      841 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
      901 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
      961 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     1021 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     1081 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     1141 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     1201 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     1261 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     1321 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     1381 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     1441 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     1501 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     1561 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     1621 ttcaaagttt ttttcttcca tttcaggtgt cgtgaggtac ctgagctcgc tagcctcgag
     1681 gatatcaaga tctggcctcg gcggccaagc ttggcaatcc ggtactgttg gtaaagccac
     1741 catggtcttc acactcgaag atttcgttgg ggactggcga cagacagccg gctacaacct
     1801 ggaccaagtc cttgaacagg gaggtgtgtc cagtttgttt cagaatctcg gggtgtccgt
     1861 aactccgatc caaaggattg tcctgagcgg tgaaaatggg ctgaagatcg acatccatgt
     1921 catcatcccg tatgaaggtc tgagcggcga ccaaatgggc cagatcgaaa aaatttttaa
     1981 ggtggtgtac cctgtggatg atcatcactt taaggtgatc ctgcactatg gcacactggt
     2041 aatcgacggg gttacgccga acatgatcga ctatttcgga cggccgtatg aaggcatcgc
     2101 cgtgttcgac ggcaaaaaga tcactgtaac agggaccctg tggaacggca acaaaattat
     2161 cgacgagcgc ctgatcaacc ccgacggctc cctgctgttc cgagtaacca tcaacggagt
     2221 gaccggctgg cggctgtgcg aacgcattct ggcgtaagtc gacttcgagc aacttgttta
     2281 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     2341 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     2401 ggatcgtcta gcatcgaaga tccaataact tcgtatagca tacattatac gaagttataa
     2461 gtagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     2521 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     2581 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     2641 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     2701 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2761 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2821 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2881 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2941 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3001 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3061 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3121 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3181 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3241 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3301 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     3361 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3421 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3481 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3541 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3601 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3661 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3721 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3781 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3841 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3901 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3961 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4021 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4081 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4141 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4201 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     4261 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4321 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4381 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4441 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4501 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4561 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4621 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     4681 gtatcactct aggcaaaata gcaccctccc gctccagaac aacatacagc gcttcacagc
     4741 ggcagcctaa cagtcagcct taccagtaaa aaagaaaacc tattaaaaaa acaccactcg
     4801 acacggcacc agctcaatca gtcacagtgt aaaaaagggc caagtgcaga gcgagtatat
     4861 ataggactaa aaaatgacgt aacggttaaa gtccacaaaa aacacccaga aaaccgcacg
     4921 cgaacctacg cccagaaacg aaagccaaaa aacccacaac ttcctcaaat cgtcacttcc
     4981 gttttcccac gttacgtaac ttcccatttt aagaaaacta caattcccaa cacatacaag
     5041 ttactccgcc ctaaaaccta cgtcacccgc cccgttccca cgccccgcgc cacgtcacaa
     5101 actccacccc ctcattatca tattggcttc aatccaaaat aaggtata
//
