Basic Vector Information
- Vector Name:
- pAAV-CMV-FLAG-FS344(opt-mouse)
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5734 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Adeno-associated virus
- Promoter:
- CMV
- 5' Primer:
- WPRE_R
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pAAV-CMV-FLAG-FS344(opt-mouse) vector Map
pAAV-CMV-FLAG-FS344(opt-mouse) vector Sequence
LOCUS . 5734 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
repeat_region complement(1..141)
/label="AAV2 ITR"
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
enhancer 156..535
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 536..739
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 773..796
/label="FLAG"
/note="FLAG® epitope tag, followed by an enterokinase
cleavage site"
misc_feature 1861..2449
/label="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
polyA_signal 2481..2957
/label="hGH poly(A) signal"
/note="human growth hormone polyadenylation signal"
repeat_region 2997..3137
/label="AAV2 ITR"
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3212..3667
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3949..4053
/label="AmpR promoter"
CDS 4054..4911
/label="AmpR"
/note="β-lactamase"
rep_origin 5085..5673
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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