LOCUS       .                       5734 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     repeat_region   complement(1..141)
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     enhancer        156..535
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        536..739
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             773..796
                     /label="FLAG"
                     /note="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    1861..2449
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    2481..2957
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     repeat_region   2997..3137
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     rep_origin      3212..3667
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3949..4053
                     /label="AmpR promoter"
     CDS             4054..4911
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      5085..5673
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgacat tgattattga ctagttatta
      181 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      241 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      301 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      361 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      421 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      481 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      541 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      601 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      661 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      721 ggtctatata agcagagctg gtaccggatc ctctagagtc gacgccacca tggattacaa
      781 ggacgacgat gacaaggcta gcatggtgag ggccaggcac cagcccggcg gcctgtgcct
      841 gctgctgctg ctgctgtgcc agttcatgga ggacaggagc gcccaggccg gcaactgctg
      901 gctgaggcag gccaagaacg gcaggtgcca ggtgctgtac aagaccgagc tgagcaagga
      961 ggagtgctgc agcaccggca ggctgagcac cagctggacc gaggaggacg tgaacgacaa
     1021 caccctgttc aagtggatga tcttcaacgg cggcgccccc aactgcatcc cctgcaagga
     1081 gacctgcgag aacgtggact gcggccccgg caagaagtgc aggatgaaca agaagaacaa
     1141 gcccaggtgc gtgtgcgccc ccgactgcag caacatcacc tggaagggcc ccgtgtgcgg
     1201 cctggacggc aagacctaca ggaacgagtg cgccctgctg aaggccaggt gcaaggagca
     1261 gcccgagctg gaggtgcagt accagggcag gtgcaagaag acctgcaggg acgtgttctg
     1321 ccccggcagc agcacctgcg tggtggacca gaccaacaac gcctactgcg tgacctgcaa
     1381 caggatctgc cccgagcccg ccagcagcga gcagtacctg tgcggcaacg acggcgtgac
     1441 ctacagcagc gcctgccacc tgaggaaggc cacctgcctg ctgggcagga gcatcggcct
     1501 ggcctacgag ggcaagtgca tcaaggccaa gagctgcgag gacatccagt gcaccggcgg
     1561 caagaagtgc ctgtgggact tcaaggtggg caggggcagg tgcagcctgt gcgacgagct
     1621 gtgccccgac agcaagagcg acgagcccgt gtgcgccagc gacaacgcca cctacgccag
     1681 cgagtgcgcc atgaaggagg ccgcctgcag cagcggcgtg ctgctggagg tgaagcacag
     1741 cggcagctgc aacagcatca gcgaggacac cgaggaggag gaggaggacg aggaccagga
     1801 ctacagcttc cccatcagca gcatcctgga gtggtagaat tcgatatcaa gcttatcgat
     1861 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     1921 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     1981 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2041 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     2101 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     2161 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     2221 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     2281 gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     2341 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     2401 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga
     2461 gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg
     2521 ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca
     2581 ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca
     2641 aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga
     2701 gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc
     2761 tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt
     2821 tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat
     2881 ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg
     2941 ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga
     3001 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     3061 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     3121 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     3181 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     3241 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3301 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3361 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3421 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3481 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3541 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     3601 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     3661 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     3721 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     3781 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     3841 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     3901 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3961 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4021 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4081 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4141 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4201 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4261 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4321 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4381 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4441 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4501 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4561 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4621 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4681 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4741 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4801 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4861 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4921 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4981 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5041 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5101 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5161 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5221 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5281 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5341 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5401 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5461 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5521 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5581 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5641 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5701 ttcctggcct tttgctggcc ttttgctcac atgt
//
