Basic Vector Information
- Vector Name:
- pBiFC-CAG-VC155
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4802 bp
- Type:
- BiFC vector
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- CAG
- 5' Primer:
- G34985-F1
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 30℃
pBiFC-CAG-VC155 vector Map
pBiFC-CAG-VC155 vector Sequence
LOCUS . 4802 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
CDS join(4788..4802,1..12)
/label="HA"
/note="HA (human influenza hemagglutinin) epitope tag"
CDS 115..366
/label="VC155"
/note="C-terminal fragment of mVenus for use in bimolecular
fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
polyA_signal complement(381..515)
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(626..642)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(650..666)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(674..704)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(719..740)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(1028..1616)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1790..2647)
/label="AmpR"
/note="β-lactamase"
promoter complement(2648..2752)
/label="AmpR promoter"
intron 3725..4741
/label="chimeric intron"
/note="chimera between introns from chicken β-actin and
rabbit β-globin"
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