LOCUS       .                       4802 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             join(4788..4802,1..12)
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             115..366
                     /label="VC155"
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
     polyA_signal    complement(381..515)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(626..642)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(650..666)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(674..704)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(719..740)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1028..1616)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1790..2647)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(2648..2752)
                     /label="AmpR promoter"
     intron          3725..4741
                     /label="chimeric intron"
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
ORIGIN
        1 ccagattacg ctcttatggc catggaggcc cgaattcggt cgaccgagat ctctcgaggt
       61 acccgtccgg cgtgcaaaat cccgaacgac ctgaaacaga aagtcatgaa ccacgacaag
      121 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
      181 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      241 gacaaccact acctgagcta ccagtccaaa ctgagcaaag accccaacga gaagcgcgat
      301 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      361 tacaagtaag cggccgcggg gatccagaca tgataagata cattgatgag tttggacaaa
      421 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
      481 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
      541 tgtttcaggt tcagggggag gtgtgggagg ttttttcgga tcctctagag tcgatctgca
      601 ggcatgctag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      661 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      721 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      781 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      841 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      901 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      961 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1021 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1081 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1141 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1201 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1261 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1321 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1381 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1441 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     1501 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1561 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1621 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1681 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1741 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1801 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1861 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1921 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1981 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2041 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2101 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2161 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2221 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2281 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2341 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2401 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2461 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2521 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     2581 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     2641 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     2701 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     2761 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     2821 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     2881 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     2941 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     3001 catcagagca gattgtactg agagtgcacc atatgcacat ttccccgaaa agtgccacct
     3061 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     3121 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     3181 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     3241 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     3301 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     3361 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     3421 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     3481 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     3541 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg
     3601 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     3661 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     3721 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
     3781 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     3841 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     3901 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     3961 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     4021 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     4081 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     4141 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     4201 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     4261 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     4321 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     4381 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     4441 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     4501 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     4561 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     4621 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     4681 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     4741 gctcctgggc aacgtgctgg ttattgtgct gtctcgggcc caccatgtac ccatacgatg
     4801 tt
//
