pCAG-hSTARR-seq_ORI vector (V017649)

LOCUS       .                       5683 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             join(5263..5683,1..437)
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      complement(630..1218)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     enhancer        1294..1673
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1675..1949
                     /label="chicken β-actin promoter"
     intron          1950..2966
                     /label="chimeric intron"
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
     intron          2987..3119
                     /label="chimeric intron"
                     /note="chimera between introns from human β-globin and
                     immunoglobulin heavy chain genes"
     promoter        3254..3284
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             3338..3994
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             4339..4641
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     polyA_signal    complement(4722..4843)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"