LOCUS       .                       5683 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             join(5263..5683,1..437)
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      complement(630..1218)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     enhancer        1294..1673
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1675..1949
                     /label="chicken β-actin promoter"
     intron          1950..2966
                     /label="chimeric intron"
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
     intron          2987..3119
                     /label="chimeric intron"
                     /note="chimera between introns from human β-globin and
                     immunoglobulin heavy chain genes"
     promoter        3254..3284
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             3338..3994
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             4339..4641
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     polyA_signal    complement(4722..4843)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gaggccctaa ggagctgact gcatttcttc ataatatggg tgatcatgtg acccggcttg
       61 accgctggga accagagttg aacgaagcca taccgaacga cgagcgtgat accacgatgc
      121 cagtagcaat ggccacaact cttcggaaac tactcactgg cgaacttctt actctagcat
      181 cacgacagca gctcatagac tggatggagg cggacaaagt agcaggacca cttcttcgct
      241 cggccctccc tgctggctgg ttcattgctg acaaatcggg ggccggtgaa cgcggctctc
      301 gcggcatcat tgctgcgctg gggcctgatg gtaagccctc acgaatcgta gtgatctaca
      361 cgacggggag tcaggccact atggacgaac gaaataggca gatcgctgag atcggtgcct
      421 cactgatcaa gcactggtaa ccactgcagt ggtttagcat ttgcggccgc tgtcagacca
      481 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
      541 ggtgaagatc ctttttgata atctcatgac atgtgagcaa aaggccagca aaaggccagg
      601 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      661 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      721 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      781 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
      841 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
      901 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
      961 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1021 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1081 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1141 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1201 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1261 aacgaaaact cacgttaagg gattttgggg cccgacattg attattgact agttattaat
     1321 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     1381 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     1441 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     1501 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     1561 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     1621 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
     1681 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
     1741 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg gggggcgcgc
     1801 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
     1861 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
     1921 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc
     1981 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
     2041 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
     2101 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
     2161 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
     2221 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
     2281 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
     2341 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
     2401 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     2461 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     2521 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     2581 aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt
     2641 gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg
     2701 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg
     2761 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
     2821 ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg
     2881 gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg
     2941 ttcatgcctt cttctttttc ctacagggtc gtgaggcact gggcaggtaa gtatcaaggt
     3001 tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt
     3061 gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg
     3121 tgtccaccct gcaggcttaa gcatggctag caaaggagaa gaactcttca ctggagttgt
     3181 cccaattctt gttgaattag attgatctag agcatgcacc ggtgatatcg cggccgcatt
     3241 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta
     3301 ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     3361 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     3421 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     3481 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     3541 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     3601 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     3661 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     3721 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     3781 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     3841 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     3901 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     3961 acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa
     4021 agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga
     4081 tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca
     4141 gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg
     4201 taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa
     4261 atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg
     4321 agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat
     4381 cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc
     4441 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg
     4501 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc
     4561 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
     4621 aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcagga
     4681 tatcgtcgac gaattcggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt
     4741 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     4801 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     4861 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     4921 ctacaaatgt ggtaaaatcg ataaggatcc gaccgatgcc cttgagagcc ttcaacccag
     4981 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
     5041 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac
     5101 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5161 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtcttgaga gacgtactag
     5221 taaccctgat aaatgcttca ataatattga aaaaggacga gtatgagcat ccaacatttt
     5281 cgtgtcgcac tcattccctt ctttgcggca ttttgcttgc ctgtttttgc acaccccgaa
     5341 acgctggtga aagtaaaaga tgctgaagat caactgggtg caagagtggg ctatatcgaa
     5401 ctggatctca atagcggcaa gatccttgag tctttccgcc ccgaagaacg attcccgatg
     5461 atgagcactt tcaaagtact gctatgtggc gcggtgttgt cccgtataga cgccgggcaa
     5521 gagcagcttg gtcgccgtat acactactca caaaacgact tggttgagta ctcgccggtc
     5581 acggaaaagc atcttacgga tggcatgacg gtaagagaat tgtgtagtgc tgccattacc
     5641 atgagcgaca acaccgcggc caacttactt ctgacaacga tcg
//
