Basic Vector Information
- Vector Name:
- pCMV-MCS1-EGFP-CAG-MCS2-Neo
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5819 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- CMV
- 5' Primer:
- pcaggs-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pCMV-MCS1-EGFP-CAG-MCS2-Neo vector Map
pCMV-MCS1-EGFP-CAG-MCS2-Neo vector Sequence
LOCUS . 5819 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
polyA_signal complement(88..143)
/label="β-globin poly(A) signal"
/note="rabbit β-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
intron complement(330..1347)
/label="chimeric intron"
/note="chimera between introns from chicken β-actin and
rabbit β-globin"
promoter 2007..2210
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2278..2994
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
polyA_signal 3120..3241
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 3270..3374
/label="AmpR promoter"
promoter 3376..3733
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 3768..4559
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase from Tn5"
polyA_signal 4794..4841
/label="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5170..5758
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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