LOCUS       .                       5819 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     polyA_signal    complement(88..143)
                     /label="β-globin poly(A) signal"
                     /note="rabbit β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     intron          complement(330..1347)
                     /label="chimeric intron"
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
     promoter        2007..2210
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2278..2994
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     polyA_signal    3120..3241
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        3270..3374
                     /label="AmpR promoter"
     promoter        3376..3733
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3768..4559
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    4794..4841
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5170..5758
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cagcatatgg gcatatgttg ccaaactcta aaccaaatac tcattctgat gttttaaatg
       61 atttgccctc ccatatgtcc ttccgagtga gagacacaaa aaattccaac acactattgc
      121 aatgaaaata aatttccttt attagccaga agtcagatgc tcaaggggct tcatgatgtc
      181 cccataattt ttggcagagg gaaaaagatc tgagctccca ccttctgata ggaagcttca
      241 gcctgcacct gctagcgagg agtgaattcc tcgaggaatt ctttgccaaa atgatgagac
      301 agcacaataa ccagcacgtt gcccaggagc tgtaggaaaa agaagaaggc atgaacatgg
      361 ttagcagagg ctctagagcc gccggtcaca cgccagaagc cgaaccccgc cctgccccgt
      421 cccccccgaa ggcagccgtc cccccgcgga cagccccgag gctggagagg gagaagggga
      481 cggcggcgcg gcgacgcacg aaggccctcc ccgcccattt ccttcctgcc ggcgccgcac
      541 cgcttcgccc cgcgcccgct agagggggtg cggcggcgcc tcccagattt cggctccgca
      601 cagatttggg acaaaggaag tccctgcgcc ctctcgcacg attaccataa aaggcaatgg
      661 ctgcggctcg ccgcgcctcg acagccgccg gcgctccggg ggccgccgcg ccccttcccc
      721 cgagccctcc ccggcccgag gcggccccgc cccgcccggc acccccacct gccgccaccc
      781 cccgcccggc acggcgagcc ccgcgccacg ccccgtacgg agccccgcac ccgaagccgg
      841 gccgtgctca gcaactcggg gaggggggtg cagggggggt tgcagcccga ccgacgcgcc
      901 cacaccccct gctcaccccc ccacgcacac accccgcacg cagcctttgt tcccctcgca
      961 gccccccccg caccgcgggg caccgccccc ggccgcgctc ccctcgcgca cactgcggag
     1021 cgcacaaagc cccgcgccgc gcccgcagcg ctcacagccg ccgggcagcg cggagccgca
     1081 cgcggcgctc cccacgcaca cacacacgca cgcacccccc gagccgctcc ccccgcacaa
     1141 agggccctcc cggagcccct caaggctttc acgcagccac agaaaagaaa caagccgtca
     1201 ttaaaccaag cgctaattac agcccggagg agaagggccg tcccgcccgc tcacctgtgg
     1261 gagtaacgcg gtcagtcaga gccggggcgg gcggcgcgag gcggcggcgg agcggggcac
     1321 ggggggaagg cagcgcgcaa cgactcccgc ccgccgcgcg cttcgctttt tatagggccg
     1381 ccgccgccgc cgcctcgcca taaaaggaaa ctttcggagc gcgccgctct gattggctgc
     1441 cgccgcacct ttccgcctcg ccccgccccg cccctcgccc cgccccgccc cgcctggggg
     1501 gggccccccc cccccccccg cccccatcgc tgcacaaaat aattaaaaaa taaataaata
     1561 caaaattggg ggtggggagg ggggggagat ggggagagtg aagcagaacg tggggctcac
     1621 ctcgaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
     1681 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
     1741 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
     1801 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
     1861 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
     1921 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
     1981 ttaataacta gtcaataatc aatgtcgtga tgcggttttg gcagtacatc aatgggcgtg
     2041 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2101 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2161 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
     2221 accgtcagat ccctgcagtc gacggtaccg cgggcccggg atccaccggt cgccaccatg
     2281 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2341 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     2401 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2461 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     2521 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2581 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2641 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2701 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     2761 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     2821 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2881 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2941 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc
     3001 ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     3061 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     3121 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3181 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3241 aagcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3301 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3361 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     3421 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3481 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3541 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     3601 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3661 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3721 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     3781 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     3841 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     3901 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     3961 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     4021 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     4081 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     4141 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     4201 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     4261 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     4321 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     4381 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     4441 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     4501 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     4561 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     4621 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     4681 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     4741 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     4801 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     4861 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     4921 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     4981 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     5041 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5101 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5161 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5221 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5281 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     5341 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5401 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5461 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5521 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5581 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5641 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5701 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5761 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
