Basic Vector Information
- Vector Name:
- pBBR1MCS2-EM7-luxCDABE
- Antibiotic Resistance:
- Kanamycin
- Length:
- 11264 bp
- Type:
- Protein expression
- Replication origin:
- pBBR1 oriV
- Host:
- Broad host
- Copy Number:
- Low
- Promoter:
- EM7
- 5' Primer:
- lac-P
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pBBR1MCS2-EM7-luxCDABE vector Map
pBBR1MCS2-EM7-luxCDABE vector Sequence
LOCUS . 11264 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
CDS 458..1249
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
protein_bind 1657..1678
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 1693..1723
/label="lac promoter"
/note="promoter for the E. coli lac operon"
promoter 1792..1810
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 1952..1999
/label="EM7 promoter"
/note="synthetic bacterial promoter"
CDS 2086..3525
/label="LuxC"
/note="LuxC fatty acid reductase"
CDS 3541..4461
/label="LuxD"
/note="LuxD acyltransferase"
CDS 4513..5592
/label="LuxA"
/note="LuxA luciferase subunit"
CDS 5610..6590
/label="LuxB"
/note="LuxB luciferase subunit"
CDS 6772..7881
/label="LuxE"
/note="LuxE"
promoter complement(8059..8077)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS complement(8813..9472)
/label="pBBR1 Rep"
/note="replication protein for the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica"
rep_origin complement(9473..10242)
/label="pBBR1 oriV"
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"
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