LOCUS       .                      11264 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             458..1249
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     protein_bind    1657..1678
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1693..1723
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     promoter        1792..1810
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1952..1999
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             2086..3525
                     /label="LuxC"
                     /note="LuxC fatty acid reductase"
     CDS             3541..4461
                     /label="LuxD"
                     /note="LuxD acyltransferase"
     CDS             4513..5592
                     /label="LuxA"
                     /note="LuxA luciferase subunit"
     CDS             5610..6590
                     /label="LuxB"
                     /note="LuxB luciferase subunit"
     CDS             6772..7881
                     /label="LuxE"
                     /note="LuxE"
     promoter        complement(8059..8077)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(8813..9472)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(9473..10242)
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca tgcgcccaat
     1561 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1621 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     1681 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     1741 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1801 tcactaaagg gaacaaaagc tgggtaccgg gccccccctc gaggtcgacg gtatcgataa
     1861 gcttgatatc gaattcaatt cctgcagggc atgccccggg catatgaagc ttggtaccgg
     1921 gatccgcggc cgcgtttaaa cccatggacg tgttgacaat taatcatcgg catagtatat
     1981 cggcatagta taatacgact cagggcccac tagtggtacc cggggatcct ctagagtcga
     2041 cctgcaggtc gacggatccg gggaattcag gcttggagga tacgtatgac taaaaaaatt
     2101 tcattcatta ttaacggcca ggttgaaatc tttcccgaag gtgatgattt agtgcaatcc
     2161 attaattttg gtgataatag tgtttacctg ccaatattga atgactctca tgtaaaaaac
     2221 attattgatt gtaatggaaa taacgaatta cggttgcata acattgtcaa ttttctctat
     2281 acggtagggc aaagatggaa aaatgaagaa tactcaagac gcaggacata cattcgtgac
     2341 ttaaaaaaat atatgggata ttcagaagaa atggctaagc tagaggccaa ttggatatct
     2401 atgattttat gttctaaagg cggcctttat gatgttgtag aaaatgaact tggttctcgc
     2461 catatcatgg atgaatggct acctcaggat gaaagttatg ttcgggcttt tccgaaaggt
     2521 aaatctgtac atctgttggc aggtaatgtt ccattatctg ggatcatgtc tatattacgc
     2581 gcaattttaa ctaagaatca gtgtattata aaaacatcgt caaccgatcc ttttaccgct
     2641 aatgcattag cgttaagttt tattgatgta gaccctaatc atccgataac gcgctcttta
     2701 tctgttatat attggcccca ccaaggtgat acatcactcg caaaagaaat tatgcaacat
     2761 gcggatgtta ttgtcgcttg gggagggcca gatgcgatta attgggcggt agagcatgcg
     2821 ccatcttatg ctgatgtgat taaatttggt tctaaaaaga gtctttgcat tatcgataat
     2881 cctgttgatt tgacgtccgc agcgacaggt gcggctcatg atgtttgttt ttacgatcag
     2941 cgagcttgtt tttctgccca aaacatatat tacatgggaa atcattatga ggaatttaag
     3001 ttagcgttga tagaaaaact taatctatat gcgcatatat taccgaatgc caaaaaagat
     3061 tttgatgaaa aggcggccta ttctttagtt caaaaagaaa gcttgtttgc tggattaaaa
     3121 gtagaggtgg atattcatca acgttggatg attattgagt caaatgcagg tgtggaattt
     3181 aatcaaccac ttggcagatg tgtgtacctt catcacgtcg ataatattga gcaaatattg
     3241 ccttatgttc aaaaaaataa gacgcaaacc atatctattt ttccttggga gtcatcattt
     3301 aaatatcgag atgcgttagc attaaaaggt gcggaaagga ttgtagaagc aggaatgaat
     3361 aacatatttc gagttggtgg atctcatgac ggaatgagac cgttgcaacg attagtgaca
     3421 tatatttctc atgaaaggcc atctaactat acggctaagg atgttgcggt tgaaatagaa
     3481 cagactcgat tcctggaaga agataagttc cttgtatttg tcccataata ggtaaaaagt
     3541 atggaaaatg aatcaaaata taaaaccatc gaccacgtta tttgtgttga aggaaataaa
     3601 aaaattcatg tttgggaaac gctgccagaa gaaaacagcc caaagagaaa gaatgccatt
     3661 attattgcgt ctggttttgc ccgcaggatg gatcattttg ctggtctggc ggaatattta
     3721 tcgcggaatg gatttcatgt gatccgctat gattcgcttc accacgttgg attgagttca
     3781 gggacaattg atgaatttac aatgtctata ggaaagcaga gcttgttagc agtggttgat
     3841 tggttaacta cacgaaaaat aaataacttc ggtatgttgg cttcaagctt atctgcgcgg
     3901 atagcttatg caagcctatc tgaaatcaat gcttcgtttt taatcaccgc agtcggtgtt
     3961 gttaacttaa gatattctct tgaaagagct ttagggtttg attatctcag tctacccatt
     4021 aatgaattgc cgaataatct agattttgaa ggccataaat tgggtgctga agtctttgcg
     4081 agagattgtc ttgattttgg ttgggaagat ttagcttcta caattaataa catgatgtat
     4141 cttgatatac cgtttattgc ttttactgca aataacgata attgggtcaa gcaagatgaa
     4201 gttatcacat tgttatcaaa tattcgtagt aatcgatgca agatatattc tttgttagga
     4261 agttcgcatg acttgagtga aaatttagtg gtcctgcgca atttttatca atcggttacg
     4321 aaagccgcta tcgcgatgga taatgatcat ctggatattg atgttgatat tactgaaccg
     4381 tcatttgaac atttaactat tgcgacagtc aatgaacgcc gaatgagaat tgagattgaa
     4441 aatcaagcaa tttctctgtc ttaaaatcta ttgagatatt ctatcactca aatagcaata
     4501 taaggactct ctatgaaatt tggaaacttt ttgcttacat accaacctcc ccaattttct
     4561 caaacagagg taatgaaacg tttggttaaa ttaggtcgca tctctgagga gtgtggtttt
     4621 gataccgtat ggttactgga gcatcatttc acggagtttg gtttgcttgg taacccttat
     4681 gtcgctgctg catatttact tggcgcgact aaaaaattga atgtaggaac tgccgctatt
     4741 gttcttccca cagcccatcc agtacgccaa cttgaagatg tgaatttatt ggatcaaatg
     4801 tcaaaaggac gatttcggtt tggtatttgc cgagggcttt acaacaagga ctttcgcgta
     4861 ttcggcacag atatgaataa cagtcgcgcc ttagcggaat gctggtacgg gctgataaag
     4921 aatggcatga cagagggata tatggaagct gataatgaac atatcaagtt ccataaggta
     4981 aaagtaaacc ccgcggcgta tagcagaggt ggcgcaccgg tttatgtggt ggctgaatca
     5041 gcttcgacga ctgagtgggc tgctcaattt ggcctaccga tgatattaag ttggattata
     5101 aatactaacg aaaagaaagc acaacttgag ctttataatg aagtggctca agaatatggg
     5161 cacgatattc ataatatcga ccattgctta tcatatataa catctgtaga tcatgactca
     5221 attaaagcga aagagatttg ccggaaattt ctggggcatt ggtatgattc ttatgtgaat
     5281 gctacgacta tttttgatga ttcagaccaa acaagaggtt atgatttcaa taaagggcag
     5341 tggcgtgact ttgtattaaa aggacataaa gatactaatc gccgtattga ttacagttac
     5401 gaaatcaatc ccgtgggaac gccgcaggaa tgtattgaca taattcaaaa agacattgat
     5461 gctacaggaa tatcaaatat ttgttgtgga tttgaagcta atggaacagt agacgaaatt
     5521 attgcttcca tgaagctctt ccagtctgat gtcatgccat ttcttaaaga aaaacaacgt
     5581 tcgctattat attagctaag gagaaagaaa tgaaatttgg attgttcttc cttaacttca
     5641 tcaattcaac aactgttcaa gaacaaagta tagttcgcat gcaggaaata acggagtatg
     5701 ttgataagtt gaattttgaa cagattttag tgtatgaaaa tcatttttca gataatggtg
     5761 ttgtcggcgc tcctctgact gtttctggtt ttctgctcgg tttaacagag aaaattaaaa
     5821 ttggttcatt aaatcacatc attacaactc atcatcctgt ccgcatagcg gaggaagctt
     5881 gcttattgga tcagttaagt gaagggagat ttattttagg gtttagtgat tgcgaaaaaa
     5941 aagatgaaat gcattttttt aatcgcccgg ttgaatatca acagcaacta tttgaagagt
     6001 gttatgaaat cattaacgat gctttaacaa caggctattg taatccagat aacgattttt
     6061 atagcttccc taaaatatct gtaaatcccc atgcttatac gccaggcgga cctcggaaat
     6121 atgtaacagc aaccagtcat catattgttg agtgggcggc caaaaaaggt attcctctca
     6181 tctttaagtg ggatgattct aatgatgtta gatatgaata tgctgaaaga tataaagccg
     6241 ttgcggataa atatgacgtt gacctatcag agatagacca tcagttaatg atattagtta
     6301 actataacga agatagtaat aaagctaaac aagagacgcg tgcatttatt agtgattatg
     6361 ttcttgaaat gcaccctaat gaaaatttcg aaaataaact tgaagaaata attgcagaaa
     6421 acgctgtcgg aaattatacg gagtgtataa ctgcggctaa gttggcaatt gaaaagtgtg
     6481 gtgcgaaaag tgtattgctg tcctttgaac caatgaatga tttgatgagc caaaaaaatg
     6541 taatcaatat tgttgatgat aatattaaga agtaccacat ggaatatacc taatagattt
     6601 cgagttgcag cgaggcggca agtgaacgaa tccccaggag catagataac tatgtgactg
     6661 gggtgagtga aagcagccaa caaagcagca gcttgaaaga tgaagggtat aaaagagtat
     6721 gacagcagtg ctgccatact ttctaatatt atcttgagga gtaaaacagg tatgacttca
     6781 tatgttgata aacaagaaat tacagcaagc tcagaaattg atgatttgat tttttcgagc
     6841 gatccattag tgtggtctta cgacgagcag gaaaaaatca gaaagaaact tgtgcttgat
     6901 gcatttcgta atcattataa acattgtcga gaatatcgtc actactgtca ggcacacaaa
     6961 gtagatgaca atattacgga aattgatgac atacctgtat tcccaacatc ggtttttaag
     7021 tttactcgct tattaacttc tcaggaaaac gagattgaaa gttggtttac cagtagcggc
     7081 acgaatggtt taaaaagtca ggtggcgcgt gacagattaa gtattgagag actcttaggc
     7141 tctgtgagtt atggcatgaa atatgttggt agttggtttg atcatcaaat agaattagtc
     7201 aatttgggac cagatagatt taatgctcat aatatttggt ttaaatatgt tatgagtttg
     7261 gtggaattgt tatatcctac gacatttacc gtaacagaag aacgaataga ttttgttaaa
     7321 acattgaata gtcttgaacg aataaaaaat caagggaaag atctttgtct tattggttcg
     7381 ccatacttta tttatttact ctgccattat atgaaagata aaaaaatctc attttctgga
     7441 gataaaagcc tttatatcat aaccggaggc ggctggaaaa gttacgaaaa agaatctctg
     7501 aaacgtgatg atttcaatca tcttttattt gatactttca atctcagtga tattagtcag
     7561 atccgagata tatttaatca agttgaactc aacacttgtt tctttgagga tgaaatgcag
     7621 cgtaaacatg ttccgccgtg ggtatatgcg cgagcgcttg atcctgaaac gttgaaacct
     7681 gtacctgatg gaacgccggg gttgatgagt tatatggatg cgtcagcaac cagttatcca
     7741 gcatttattg ttaccgatga tgtcgggata attagcagag aatatggtaa gtatcccggc
     7801 gtgctcgttg aaattttacg tcgcgtcaat acgaggacgc agaaagggtg tgctttaagc
     7861 ttaaccgaag cgtttgatag ttgatgacct gcaggcatgc aagcttgcgg ccgcgggccc
     7921 catggatcga tagctggtcg acacaatctg ccctttcgaa agatcccaac gaaaagcgtg
     7981 accacatggt ccttcttgag tttgtaacgg atccactagt tctagagcgg ccgccaccgc
     8041 ggtggagctc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     8101 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     8161 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8221 tgcgcagcct gaatggcgaa tggaaattgt aagcgttaat attttgttaa aattcgcgtt
     8281 aaatttttgt taaatcagct cattttttaa ccaataggcc gactgcgatg agtggcaggg
     8341 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga
     8401 ataagtgata ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg
     8461 gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga
     8521 ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc
     8581 tccccgtgga ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa
     8641 aaacggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct
     8701 ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcga ggcggctaca
     8761 gccgatagtc tggaacagcg cacttacggg ttgctgcgca acccaagtgc taccggcgcg
     8821 gcagcgtgac ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa cacggctcat
     8881 cgggcatcgg caggcgctgc tgcccgcgcc gttcccattc ctccgtttcg gtcaaggctg
     8941 gcaggtctgg ttccatgccc ggaatgccgg gctggctggg cggctcctcg ccggggccgg
     9001 tcggtagttg ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg ccatgcccca
     9061 acagcgattc gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac accgacaggc
     9121 gcaactggtc gcggggctgg ccccacgcca cgcggtcatt gaccacgtag gccgacacgg
     9181 tgccggggcc gttgagcttc acgacggaga tccagcgctc ggccaccaag tccttgactg
     9241 cgtattggac cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc tggctgacca
     9301 ccacggcgtt ctggtggccc atctgcgcca cgaggtgatg cagcagcatt gccgccgtgg
     9361 gtttcctcgc aataagcccg gcccacgcct catgcgcttt gcgttccgtt tgcacccagt
     9421 gaccgggctt gttcttggct tgaatgccga tttctctgga ctgcgtggcc atgcttatct
     9481 ccatgcggta gggtgccgca cggttgcggc accatgcgca atcagctgca acttttcggc
     9541 agcgcgacaa caattatgcg ttgcgtaaaa gtggcagtca attacagatt ttctttaacc
     9601 tacgcaatga gctattgcgg ggggtgccgc aatgagctgt tgcgtacccc ccttttttaa
     9661 gttgttgatt tttaagtctt tcgcatttcg ccctatatct agttctttgg tgcccaaaga
     9721 agggcacccc tgcggggttc ccccacgcct tcggcgcggc tccccctccg gcaaaaagtg
     9781 gcccctccgg ggcttgttga tcgactgcgc ggccttcggc cttgcccaag gtggcgctgc
     9841 ccccttggaa cccccgcact cgccgccgtg aggctcgggg ggcaggcggg cgggcttcgc
     9901 cttcgactgc ccccactcgc ataggcttgg gtcgttccag gcgcgtcaag gccaagccgc
     9961 tgcgcggtcg ctgcgcgagc cttgacccgc cttccacttg gtgtccaacc ggcaagcgaa
    10021 gcgcgcaggc cgcaggccgg aggcttttcc ccagagaaaa ttaaaaaaat tgatggggca
    10081 aggccgcagg ccgcgcagtt ggagccggtg ggtatgtggt cgaaggctgg gtagccggtg
    10141 ggcaatccct gtggtcaagc tcgtgggcag gcgcagcctg tccatcagct tgtccagcag
    10201 ggttgtccac gggccgagcg aagcgagcca gccggtggcc gctcgcggcc atcgtccaca
    10261 tatccacggg ctggcaaggg agcgcagcga ccgcgcaggg cgaagcccgg agagcaagcc
    10321 cgtagggcgc cgcagccgcc gtaggcggtc acgactttgc gaagcaaagt ctagtgagta
    10381 tactcaagca ttgagtggcc cgccggaggc accgccttgc gctgcccccg tcgagccggt
    10441 tggacaccaa aagggagggg caggcatggc ggcatacgcg atcatgcgat gcaagaagct
    10501 ggcgaaaatg ggcaacgtgg cggccagtct caagcacgcc taccgcgagc gcgagacgcc
    10561 caacgctgac gccagcagga cgccagagaa cgagcactgg gcggccagca gcaccgatga
    10621 agcgatgggc cgactgcgcg agttgctgcc agagaagcgg cgcaaggacg ctgtgttggc
    10681 ggtcgagtac gtcatgacgg ccagcccgga atggtggaag tcggccagcc aagaacagca
    10741 ggcggcgttc ttcgagaagg cgcacaagtg gctggcggac aagtacgggg cggatcgcat
    10801 cgtgacggcc agcatccacc gtgacgaaac cagcccgcac atgaccgcgt tcgtggtgcc
    10861 gctgacgcag gacggcaggc tgtcggccaa ggagttcatc ggcaacaaag cgcagatgac
    10921 ccgcgaccag accacgtttg cggccgctgt ggccgatcta gggctgcaac ggggcatcga
    10981 gggcagcaag gcacgtcaca cgcgcattca ggcgttctac gaggccctgg agcggccacc
    11041 agtgggccac gtcaccatca gcccgcaagc ggtcgagcca cgcgcctatg caccgcaggg
    11101 attggccgaa aagctgggaa tctcaaagcg cgttgagacg ccggaagccg tggccgaccg
    11161 gctgacaaaa gcggttcggc aggggtatga gcctgcccta caggccgccg caggagcgcg
    11221 tgagatgcgc aagaaggccg atcaagccca agagacggcc cgag
//
